Immune effect of heparin-binding hemagglutinin adhesin on pulmonary tuberculosis and extra-pulmonary tuberculosis

Abstract

Abstract: ObjectiveTo investigate the immune effect of heparin-binding hemagglutinin adhesin (HBHA) on different states of tuberculosis infection and its diagnosis value. MethodsNative HBHA were used to stimulate peripheral blood mononuclear cells (PBMCs) from the different cases including negative control, latent tuberculosis (LTB), pulmonary tuberculosis, and extra-pulmonary tuberculosis, the interferon-gamma (IFN-γ) in the supernatant of culture was detected by ELISA method. Nonparametric test was used to analyse the data. ResultsThe median level of HBHA-specific IFN-γ in the four groups were 49.5 pg/ml, 781.9 pg/ml, 341.8 pg/ml, 214.4 pg/ml, respectively. The IFN-γ level in LTB group was higher than other groups. The IFN-γ level in pulmonary tuberculosis group and extra-pulmonary tuberculosis group after stimulation with HBHA showed no significant difference (U＝1901.5，P＝0.448). ConclusionsHBHA-specific IFN-g release may identify tuberculosis infection cases from normal cases.

Key words: Tuberculosis,pulmonary/immunology, Tuberculosis/immunology, Lectins, Interferon type Ⅱ

Nie Lihui, Sun Zhaogang, Zhang Xuxia, Li Chanyou. Immune effect of heparin-binding hemagglutinin adhesin on pulmonary tuberculosis and extra-pulmonary tuberculosis[J]. Chinese Journal of Antituberculosis, 2009, 31(9): 544-547.

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