Abstract: Objective To study possible role of HLA-DR gene haplotype in incidence of pulmonary tuberculosis among Han nationality of southern Chinese.Methods Case-control study was implemented and haplotype frequencies(HF) of DR53-DRB1、DR52-DRB1 and DR51-DRB1 were compared and relative risks (RR)were calculated by using PCR-SSP to detect 23 alleles of HLA-DR gene sites of 110 tuberculosis cases and 101 controls who come from Guangdong, Guangxi, Hunan, Hubei,Jiangxi and Fujian.Results Frequency of DR16-DR52 haplotype in pulmonary tuberculosis cases is strikingly higher than that in controls (0.01< P <0.05) and its RR is 3.10.Frequencies of DR1-DR53、DR1-DR52、DR13.3-DR53、DR12-DR53、DR13.3-DR52、 DR7-DR52、DR9-DR52 haplotype in pulmonary tuberculosis cases is remarkably lower than that in controls (for DR1-DR53、DR1-DR52、DR13.3-DR53、DR12-DR53, P <0.01, for DR13.3-DR52、 DR7-DR52、DR9-DR52, 0.01< P <0.05) and their RRs are 0.29、0.26、0.38、0.52、0.25、0.10 and 0.42 respectively.Conclusion DR12-DR53、DR7-DR52、DR9-DR52 haplotype are potential relative to incidence of pulmonary tuberculosis among Han nationality of southern Chinese. However, difference of frequencies of DR16-DR52、DR1-DR53、DR1-DR52、DR13.3-DR53、DR13.3-DR52 haplotype between pulmonary tuberculosis cases and controls may be caused by remarkable differences of gene frequency.

Key words: Pulmonary tuberculosis (PTB), HLA, Haplotype, PCR-SSP