Abstract: Objective To compare influence of various DNA fixation methods on hybridization of PCR products of M.tuberculosis.Methods PCR products of M.tuberculosis were obtained,then diluted into different concentration and added to the nylon membrane.PCR products on the nylon membrane were fixed with UV crosslinking,oven baking and alkali fixation method,then hybridized and analysed.Results It was found that DNA fixation method had a great influence on detection of PCR products of M.tuberculosis.Alkali fixation had a good sensitivity and reproducibility.Conclusion All the results suggested that PCR laboratory of M.tuberculosis could consider using alkali fixation to PCR products,then hybridized and detected.

Key words: DNA fixation, M.tuberculosis, PCR, Hybridization