Abstract: Objective To study the impact of store temperature,time and wash method of sputum specimens contained CPB on the separate ratio of M.tuberculosis.Method The sputum specimens contained CPB were collected and stored in different temperature,time and prepared with two wash methods,to observe and compare the separate ratio of M.tuberculosis.Result The sputum samples contained CPB are steady if stored at beyond 18℃,compare with the condition under 18℃.The positive ratio exists marked difference( P<0.05).If the samples are saved for 1～3 weeks,the positive ratio is relatively higher,and saved for over 4 weeks,the postive ratio declines boviously.There exist marked difference( P<0.05)compared with one week.Conclusion The positive ratio of M.tuberculosis of the samples contained CPB is related to the sample reseving temperature and period.

Key words: CPB, M.tuberculosis, Sputum samples