Abstract: Objective To predict the epitopes of Mycobacterium tuberculosis Rv1419 protein. Methods The amino acid sequence of Rv1419 protein was input and predicted B cell and T cell epitopes using multi-parameters including the secondary structure, hydrophilicity, antigenicity, accessibility, flexibility, charge distribution by Protean software of DNAStar software package. Results The Rv1419 protein has rich secondary structure and multiple sections with higher antigenicity. There were a few potential B cell epitopes at 67－70, 72－82, 142－154, 131－137 amino acid residues or nearby, these epitopes had better antigenicity, contained beta angle and irregular coil structure, present in the surface probability and had larger flexibility. There also were more potential T cell epitopes of the protein containing at 5－15, 18－22, 29－32, 43－55, 58－62, 64－68, 86－97, 99－109, 110－114, 117－123, 126－128, 136－140 amino acid residues or nearby. Conclusion Mycobacterium tuberculosis Rv1419 protein is a T-cell-epitope dominant antigen, B cell epitopes also exists, which will lay the foundation for its further study and application.

Key words: Epitopes, Bacterial proteins, Protein structure, secondary, Mycobacterium tuberculosis