Chinese Journal of Antituberculosis ›› 2021, Vol. 43 ›› Issue (2): 159-165.doi: 10.3969/j.issn.1000-6621.2021.02.011
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WANG Xiu-jun, LIU Qiu-yue, CHEN Xiao-feng, YU Lei, MA Yan, HAN Fen()
Received:
2020-10-23
Online:
2021-02-10
Published:
2021-02-03
Contact:
HAN Fen
E-mail:lmegi@163.com
WANG Xiu-jun, LIU Qiu-yue, CHEN Xiao-feng, YU Lei, MA Yan, HAN Fen. Study on plasma proteomics of patients with secondary pulmonary tuberculosis based on label-free quantitative technology[J]. Chinese Journal of Antituberculosis, 2021, 43(2): 159-165. doi: 10.3969/j.issn.1000-6621.2021.02.011
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GO ID | 分子功能名称 | 富集的差异蛋白个数 | 构成比(%) | |
---|---|---|---|---|
GO:0003676 | 核酸结合(nucleic acid binding) | 127 | 9.32 | |
GO:0003774 | 运动活动(motor activity) | 26 | 1.91 | |
GO:0003824 | 催化活性(catalytic activity) | 20 | 1.47 | |
GO:0004386 | helicase activity | 5 | 0.37 | |
GO:0004871 | 信号传感器活动(signal transducer activity) | 42 | 3.08 | |
GO:0004872 | 受体活性(receptor activity) | 40 | 2.94 | |
GO:0005198 | 结构分子活性(structural molecule activity) | 30 | 2.20 | |
GO:0005215 | 转运活性(transporter activity) | 31 | 2.27 | |
GO ID | 分子功能名称 | 富集的差异蛋白个数 | 构成比(%) | |
GO:0005488 | 结合(binding) | 406 | 29.79 | |
GO:0005515 | 蛋白质结合(protein binding) | 338 | 24.80 | |
GO:0008565 | 蛋白质转运体活性(protein transporter activity) | 4 | 0.29 | |
GO:0009055 | 电子载流子活性(electron carrier activity) | 4 | 0.29 | |
GO:0015075 | 离子跨膜转运活性(ion transmembrane transporter activity) | 15 | 1.10 | |
GO:0015267 | 通道活动(channel activity) | 12 | 0.88 | |
GO:0016209 | 抗氧化活性(antioxidant activity) | 4 | 0.29 | |
GO:0016301 | 激酶活性(kinase activity) | 43 | 3.16 | |
GO:0016491 | 氧化还原酶活性(oxidoreductase activity) | 19 | 1.39 | |
GO:0016740 | 转移酶活性(transferase activity) | 73 | 5.36 | |
GO:0016787 | 水解酶活性(hydrolase activity) | 74 | 5.43 | |
GO:0016829 | 裂解酶活性(lyase activity) | 7 | 0.51 | |
GO:0016853 | 异构酶活性(isomerase activity) | 7 | 0.51 | |
GO:0016874 | 连接酶活性(ligase activity) | 4 | 0.29 | |
GO:0030234 | 酶调节活性(enzyme regulator activity) | 32 | 2.35 |
GO ID | 生物进程名称 | 富集的差异蛋白个数 | 构成比(%) |
---|---|---|---|
GO:0006139 | 含核碱化合物代谢过程(nucleobase-containing compound metabolic process) | 164 | 5.18 |
GO:0006520 | 细胞氨基酸代谢过程(cellular amino acid metabolic process) | 10 | 0.32 |
GO:0006810 | 运输(transport) | 160 | 5.05 |
GO:0006928 | 细胞或亚细胞成分的运动(movement of cell or subcellular component) | 82 | 2.59 |
GO:0006944 | 膜融合(membrane fusion) | 7 | 0.22 |
GO:0007154 | 细胞通讯(cell communication) | 169 | 5.34 |
GO:0007275 | 多细胞生物发育(multicellular organism development) | 146 | 4.61 |
GO:0007610 | 行为(behavior) | 19 | 0.60 |
GO:0008152 | 代谢过程(metabolic process) | 352 | 11.11 |
GO:0008219 | 细胞死亡(cell death) | 70 | 2.21 |
GO:0009056 | 分解代谢过程(catabolic process) | 62 | 1.96 |
GO:0009058 | 生物合成过程(biosynthetic process) | 167 | 5.27 |
GO:0009987 | 细胞过程(cellular process) | 467 | 14.75 |
GO:0030154 | 细胞分化(cell differentiation) | 121 | 3.82 |
GO:0032501 | 大分子代谢过程(multicellular organismal process) | 163 | 5.15 |
GO:0043062 | 细胞外结构组织(extracellular structure organization) | 22 | 0.69 |
GO:0043170 | 大分子代谢过程(macromolecule metabolic process) | 298 | 9.41 |
GO:0046903 | 分泌物(secretion) | 57 | 1.80 |
GO:0050789 | 生物过程调控(regulation of biological process) | 336 | 10.61 |
GO:0050896 | 刺激反应(response to stimulus) | 235 | 7.42 |
GO:0051704 | 多生物过程(multi-organism process) | 60 | 1.89 |
GO ID | 细胞定位名称 | 富集的差异蛋白个数 | 构成比(%) |
---|---|---|---|
GO:0005576 | 胞外区(extracellular region) | 129 | 7.32 |
GO:0005578 | 蛋白质细胞外基质(proteinaceous extracellular matrix) | 30 | 1.70 |
GO:0005615 | 细胞外间隙(extracellular space) | 61 | 3.46 |
GO:0005622 | 细胞内(intracellular) | 389 | 22.07 |
GO:0005623 | 细胞(cell) | 388 | 22.01 |
GO:0005634 | 核(nucleus) | 207 | 11.74 |
GO:0005694 | 染色体 (chromosome) | 27 | 1.53 |
GO:0005737 | 细胞质(cytoplasm) | 298 | 16.90 |
GO:0009986 | 细胞表面(cell surface) | 17 | 0.96 |
GO:0016020 | 膜(membrane) | 217 | 12.31 |
通路名称 | 通路编码 | 通路数 | 链接 | |||
---|---|---|---|---|---|---|
上调通路 | ||||||
背腹轴形成通路(dorso-ventral axis formation) | hsa04320 | 8 | http://www.genome.jp/kegg-bin/show_pathway?hsa04320/hsa:6654%09red/hsa:440822%09red/hsa:143689%09red/hsa:4853%09red/hsa:673%09red | |||
黏着斑通路(focal adhesion) | hsa04510 | 21 | http://www.genome.jp/kegg-bin/show_pathway?hsa04510/hsa:3480%09red/hsa:3918%09red/hsa:7448%09red/hsa:5582%09red/hsa:1286%09red/hsa:673%09red/hsa:57144%09red/hsa:5293%09red/hsa:9564%09red/hsa:2335%09red/hsa:6654%09red/hsa:81%09red/hsa:284217%09red/hsa:7148%09red | |||
癌症蛋白多糖通路(proteoglycans in cancer) | hsa05205 | 20 | http://www.genome.jp/kegg-bin/show_pathway?hsa05205/hsa:2817%09red/hsa:7477%09red/hsa:5293%09red/hsa:5582%09red/hsa:7099%09red/hsa:6194%09red/hsa:7448%09red/hsa:2335%09red/hsa:6654%09red/hsa:2065%09red/hsa:673%09red/hsa:3480%09red/hsa:3236%09red/hsa:3710%09red/hsa:7484%09red | |||
血小板活化通路(platelet activation) | hsa04611 | 13 | http://www.genome.jp/kegg-bin/show_pathway?hsa04611/hsa:3710%09red/hsa:2243%09red/hsa:5293%09red/hsa:2244%09red/hsa:111%09red/hsa:5028%09red/hsa:4067%09red | |||
ECM受体相互作用(ECM-receptor interaction) | hsa04512 | 10 | http://www.genome.jp/kegg-bin/show_pathway?hsa04512/hsa:3918%09red/hsa:1286%09red/hsa:7448%09red/hsa:2335%09red/hsa:284217%09red/hsa:7148%09red | |||
补体途径凝血通路(complement and coagulation cascades) | hsa04610 | 9 | http://www.genome.jp/kegg-bin/show_pathway?hsa04610/hsa:3827%09red/hsa:2243%09red/hsa:7448%09red/hsa:2244%09red/hsa:2157%09red/hsa:717%09red/hsa:716%09red | |||
癌症小RNA通路(MicroRNAs in cancer) | hsa05206 | 14 | http://www.genome.jp/kegg-bin/show_pathway?hsa05206/hsa:1789%09red/hsa:5582%09red/hsa:3236%09red/hsa:6654%09red/hsa:2065%09red/hsa:4853%09red/hsa:324%09red/hsa:7148%09red | |||
非洲嗜睡病通路(African trypanosomiasis) | hsa05143 | 6 | http://www.genome.jp/kegg-bin/show_pathway?hsa05143/hsa:3043%09red/hsa:3040%09red/hsa:5582%09red/hsa:3250%09red | |||
通路名称 | 通路编码 | 通路数 | 链接 | |||
mTOR 信号通路(mTOR signaling pathway) | hsa04150 | 13 | http://www.genome.jp/kegg-bin/show_pathway?hsa04150/hsa:7477%09red/hsa:3480%09red/hsa:5582%09red/hsa:673%09red/hsa:6194%09red/hsa:5293%09red/hsa:27330%09red/hsa:6654%09red/hsa:7484%09red/hsa:6520%09red/hsa:6249%09red | |||
ErbB 信号通路(ErbB signaling pathway) | hsa04012 | 10 | http://www.genome.jp/kegg-bin/show_pathway?hsa04012/hsa:685%09red/hsa:5582%09red/hsa:673%09red/hsa:57144%09red/hsa:5293%09red/hsa:6654%09red/hsa:2065%09red | |||
EGFR酪氨酸激酶抑制剂抵抗通路(EGFR tyrosine kinase inhibitor resistance) | hsa01521 | 9 | http://www.genome.jp/kegg-bin/show_pathway?hsa01521/hsa:3480%09red/hsa:5582%09red/hsa:673%09red/hsa:6194%09red/hsa:5293%09red/hsa:6654%09red/hsa:2065%09red | |||
长时程抑制通路(Long-term depression) | hsa04730 | 8 | http://www.genome.jp/kegg-bin/show_pathway?hsa04730/hsa:3480%09red/hsa:673%09red/hsa:4067%09red/hsa:5582%09red/hsa:3710%09red | |||
阿米巴通路(Amoebiasis) | hsa05146 | 10 | http://www.genome.jp/kegg-bin/show_pathway?hsa05146/hsa:3918%09red/hsa:5582%09red/hsa:1286%09red/hsa:7099%09red/hsa:5293%09red/hsa:2335%09red/hsa:81%09red/hsa:284217%09red | |||
内分泌抵抗通路(endocrine resistance) | hsa01522 | 10 | http://www.genome.jp/kegg-bin/show_pathway?hsa01522/hsa:5293%09red/hsa:673%09red/hsa:3480%09red/hsa:6654%09red/hsa:111%09red/hsa:4853%09red | |||
肾细胞癌通路(renal cell carcinoma) | hsa05211 | 8 | http://www.genome.jp/kegg-bin/show_pathway?hsa05211/hsa:6654%09red/hsa:57144%09red/hsa:5293%09red/hsa:2271%09red/hsa:673%09red | |||
PI3K-Akt信号通路(PI3K-Akt signaling pathway) | hsa04151 | 21 | http://www.genome.jp/kegg-bin/show_pathway?hsa04151/hsa:7533%09red/hsa:3918%09red/hsa:7448%09red/hsa:1286%09red/hsa:7099%09red/hsa:6194%09red/hsa:5293%09red/hsa:2335%09red/hsa:6654%09red/hsa:3815%09red/hsa:284217%09red/hsa:3480%09red/hsa:7148%09red | |||
磷脂酰肌醇信号系统(phosphatidylinositol signaling system) | hsa04070 | 9 | http://www.genome.jp/kegg-bin/show_pathway?hsa04070/hsa:5293%09red/hsa:5287%09red/hsa:5582%09red/hsa:3710%09red | |||
下调通路 | ||||||
肌动蛋白细胞骨架的调节通路(regulation of actin cytoskeleton) | hsa04810 | 14 | http://www.genome.jp/kegg-bin/show_pathway?hsa04810/hsa:2934%09red/hsa:673%09red/hsa:57144%09red/hsa:5293%09red/hsa:79784%09red/hsa:9564%09red/hsa:2335%09red/hsa:6654%09red/hsa:81%09red/hsa:324%09red |
[1] | World Health Organization. Global tuberculosis report 2020. Geneva: World Health Organization, 2020. |
[2] | Zhang X, Liang Z, Zhang Y, et al. Comprehensive analysis of long non-coding RNAs expression pattern in the pathogenesis of pulmonary tuberculosis. Genomics, 2020,112(2):1970-1977. doi: 10.1016/j.ygeno.2019.11.009. |
[3] | Zetter M, Barrios-Payán J, Mata-Espinosa D, et al. Involvement of Vasopressin in the Pathogenesis of Pulmonary Tuberculosis: A New Therapeutic Target? Front Endocrinol (Lausanne), 2019,10:351. doi: 10.3389/fendo.2019.00351. |
[4] | Nahnsen S, Bielow C, Reinert K, et al. Tools for label-free peptide quantification. Mol Cell Proteomics, 2013,12(3):549-556. doi: 10.1074/mcp.R112.025163. |
[5] | Ghafourian S, Sekawi Z, Raftari M, et al. Application of proteomics in lab diagnosis. Clin Lab, 2013,59(5/6):465-474. |
[6] | 中华人民共和国国家卫生和计划生育委员会. WS 288—2017 肺结核诊断. 2017-11-09. |
[7] | Hanash S. A call for a fresh new look at the plasma proteome. Proteomics Clin Appl, 2012,6(9/10):443-446. doi: 10.1002/prca.201200052. |
[8] | Claydon AJ, Beynon R. Proteome dynamics: revisiting turnover with a global perspective. Mol Cell Proteomics, 2012,11(12):1551-1565. doi: 10.1074/mcp.O112.022186. |
[9] | Garbis SD, Townsend PA. Proteomics of human prostate cancer biospecimens: the global, systems-wide perspective for protein markers with potential clinical utility. Expert Rev Proteomics, 2013,10(4):337-354. doi: 10.1586/14789450.2013.827408. |
[10] | Lee PL, Jerng JS, Chang YL, et al. Patient mortality of active pulmonary tuberculosis requiring mechanical ventilation. Eur Respir J, 2003,22(1):141-147. doi: 10.1183/09031936.03.00038703. |
[11] | Wallis RS, Pai M, Menzies D, et al. Biomarkers and diagnostics for tuberculosis: progress, needs, and translation into practice. Lancet, 2010,375(9729):1920-1937. doi: 10.1016/S0140-6736(10)60359-5. |
[12] | 吴艺舟, 严雨欣, 孙杰. 黏着斑信号通路调控肿瘤上皮间质转化的研究进展. 医学理论与实践, 2015,28(19):2601-2604. |
[13] | Nishi K, Maruyama T, Halsall HB, et al. Binding of alpha1-acid glycoprotein to membrane results in a unique structural change and ligand release. Biochemistry, 2004,43(32):10513-10519. doi: 10.1021/bi0400204. |
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