Abstract: Objective To explore the diagnostic value of Mycobacterium tuberculosis fusion antigen 38F and 64F in detecting IgG, IgM and IgA antibody in serum of patients with active pulmonary tuberculosis. Methods By using enzyme-linked immuno sorbent assay (ELISA) method, IgG, IgM and IgA antibody levels in serum of 223 patients with active pulmonary tuberculosis were detected with the Mycobacterium tuberculosis seven immune-dominat peptide fusion antigen 38F and 64F. Patients with active pulmonary tuberculosis were divided into three groups. The first group was co-positive cases of smear and culture examination (n=86). The second group was smear-negative and culture-positive (n=51). The third group was co-negative cases of smear and culture examination (n=86). Results The joint detection rate of IgG, IgM and IgA was 79.07% （68/86） for the first group, 62.75% （32/51） for the second group and 69.77% （60/86） for the third group. The detection rate of all samples by serological method was 71.75% （160/223）. Conclusion Mycobacterium tuberculosis fusion antigen 38F and 64F have high assistant diagnostic values to active pulmonary tuberculosis, and the detection rate is increased significantly through joint detection of IgG, IgM and IgA antibody.

Key words: Tuberculosis, pulmonary/diagnosis, Antibodies, bacterial, Bacterial protein, Serologic tests