Research progress of phenotype detection of Mycobacterium tuberculosis drug susceptibility

Abstract

Abstract: The epidemic situation of global drug-resistant tuberculosis is still severe, particularly in China. The phenotype detection of Mycobacterium tuberculosis drug susceptibility still plays an important role in the diagnosis of drug-resistant tuberculosis. This paper discussed the research progress of various phenotypic drug susceptibility testing methods at home and abroad in recent years, summarized the sensitivities, specificities of various methods and their advantages and disadvantages, which will provide a basis for selecting drug susceptibility test methods in clinical laboratories according to the different demand.

Key words: Mycobacterium tuberculosis, Microbial sensitivity tests, Phenotype

SHEN Xiao-na, ZHAO Yan-lin, XIAO He-ping. Research progress of phenotype detection of Mycobacterium tuberculosis drug susceptibility[J]. Chinese Journal of Antituberculosis, 2013, 35(6): 463-467.

References

［1］WHO/IUATLD global working group on anti-tuberculosis drug resistance surveillance. Guidelines for surveillance of drug resistance in tuberculosis.Geneva: WHO, 1997.
［2］刘宇红，姜广路，赵立平，等．第四次全国结核病流调分枝杆菌药敏试验的方法学探讨．中国防痨杂志，2003，25（1）:16-20．
［3］赵玉玲.2种方法检测结核分枝杆菌对10种药物耐药性的比较.临床检验杂志,2004，22(5):358-359.
［4］武洁，桂晓红，李静，等.比例法与绝对浓度法检测结核分枝杆菌药敏试验的比较.中华检验医学杂志，2011，34(2):137-138.
［5］Caviedes L, Lee TS, Gilman RH, et a1．Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures. The Tuberculosis Working Group in Peru.Clin Micmbiol，2000，38(3)：1203-1208．
［6］Makamure B, Mhaka J, Makumbirofa S, et al. Microscopic-observation drug-susceptibility assay for the diagnosis of drug-resistant tuberculosis in Harare, Zimbabwe. PLoS One, 2013,8(2): e55872.
［7］Rasslan O, Hafez SF, Hashem M, et al. Microscopic observation drug susceptibility assay in the diagnosis of multidrug-resistant tuberculosis. Int J Tuberc Lung Dis, 2012, 16(7):941-946.
［8］陆俊梅，王洁，黄晓辰，等. 显微镜观察药物敏感性检测技术在痰标本直接药敏试验中的应用.中华预防医学杂志，2011，45(1):21-25.
［9］Verma JS,Rawat D,Hasan A, et al. The use of E-test for the drug susceptibility testing of Mycobacterium tuberculosis-A solution or an illusion? Indian J Med Microbiol, 2010, 28(1):30-33.
［10］Pfyffer GE, Bonato DA, Ebrahimzadeh A, et al. Multicenter laboratory validation of susceptibility testing of Mycobacterium tuberculosis against classical second-line and newer antimicro-bial drugs by using the radiometric BACTEC 460 technique and the proportion method with solid media. J Clin Microbiol，1999，37(10):3179-3186.
［11］张娟，蒋俊，张红，等，MGIT960与罗氏培养法在结核分枝杆菌培养及药敏试验中的比对分析.中国防痨杂志，2011，33(6): 361-365.
［12］Siddiqi S，Ahmed A,Asif S，et al. Direct drug susceptibility testing of Mycobacterium tuberculosis for rapid detection of multidrug resistance using the Bactec MGIT 960 system: a multicenter study. J Clin Microbiol，2012, 50(2): 435-440.
［13］Otu J, Antonio M, Cheung YB,et al. Comparative evaluation of BACTEC MGIT 960 with BACTEC 9000 MB and LJ for isolation of mycobacteria in The Gambia. J Infect Dev Ctries，2008，2(3):200-205.
［14］Wilson ML, Stone BL, Hildred MV,et al. Comparison of recovery rates for mycobacteria from BACTEC 12B vials, Middlebrook 7H11-selective 7H11 biplates, and Lowenstein Jen-sen slants in a public health mycobacteriology laboratory. J Clin Microbiol，1995，33(9):2516-2518.
［15］Nair D,Capoor MR,Rawat D,et al. Standardization of first and second-line antitubercular susceptibility testing using BacT Alert 3D system: a report from a tertiary care centre in India.Braz J Infect Dis，2009,13(6):422-426.
［16］Baylan O，Kisa O，Albay A，et a1．Evaluation of a new automated, rapid, colorimetric culture system using solid medium for laboratory diagnosis of tuberculosis and determination of anti-tuberculosis drug susceptibility. Int Tuberc Lung Dis,2004,8(6)：772-777．
［17］Akihisa T, Franzblau SG, Ukiya M,et al. Antitubercular activity of triterpenoids from Asteraceae flowers. Biol Pharm Bull，2005，28(1):158-160.
［18］Sriram D, Yogeeswari P, Madhu K. Synthesis and in vitro and in vivo antimycobacterial activity of isonicotinoyl hydrazones. Bioorg Med Chem Lett，2005，15(20):4502-4505.
［19］Yajko DM, Madej JJ, Lancaster MV, et al. Colorimetric method for determining MICs of antimicrobial agents for Mycobacterium tuberculosis. J Clin Microbiol，1995,33(9): 2324-2327.
［20］Farnia P, Mohammadi F, Mirsaedi M, et al．Bacteriological follow-up of pulmonary tuberculosis treatment: a study with a simple colorimetric assay. Microbes Infect，2004，6(11)：972-976.
［21］李桂莲，张敬蕊，赵秀芹，等. 微孔板Alamar Blue显色法检测结核分枝杆菌临床分离株利福平和异烟肼耐药性研究. 中国人兽共患病学报, 2012, 28(4):319-322.
［22］Palomino JC, Martin A, Camacho M，et al. Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis. Antimicrob Agents Chemother, 2002, 46(8):2720-2722.
［23］Martin A,Camacho M, Portaels F, et a1.Resazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs：rapid，simple，and inexpensive method．Antimicrob Agents Chemother,2003,47(11)：3616-3619.
［24］张宗德，邢爱英，刘忠泉，等. 噻唑蓝和刃天青法在一线抗结核药物药敏试验中的应用. 中华检验医学杂志,2008, 31(9)：989-992.
［25］Mshana RN, Tadesse G, Abate G,et al. Use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide for rapid detection of rifampin-resistant Mycobacterium tuberculosis. J Clin Microbiol，1998，36(5): 1214-1219.
［26］Pontino MV, Di Giulio B, Fernández C,et al. Evaluation of a colorimetric micromethod for determining the minimal inhibitory concentration of antibiotics against Mycobacterium tuberculosis. Rev Argent Microbiol，2006，38(3):145-151.
［27］Ferrari Mde L, Telles MA, Ferrazoli L,et al. Susceptibility of Mycobacterium tuberculosis to first-line antimycobacterial agents in a Brazilian hospital: assessing the utility of the tetrazolium (MTT) microplate assay.Mem Inst Oswaldo Cruz，2010，105(5): 661-664.
［28］Morcillo N, Di Giulio B, Testani B,et al. A microplate indicator-based method for determining the susceptibility of multidrug-resistant Mycobacterium tuberculosis to antimicrobial agents. Int J Tuberc Lung Dis，2004，8(2):253-259.
［29］Coban AY, Uzun M, Akgunes A,et al. Comparative evaluation of the microplate nitrate reductase assay and the rezasurin microtitre assay for the rapid detection of multidrug resistant Mycobacterium tuberculosis clinical isolates. Mem Inst Oswaldo Cruz, 2012, 107(5): 578-581.
［30］Syre H，Valvatne H，Sandven P，et al．Evaluation of the nitrate-based colorimetric method for testing the susceptibility of Mycobacterium tuberculosis to streptomycin and ethambutol in liquid cultures. J Antimicrob Chemother，2006，57(5)：987-991．
［31］戚超群，杨贵发.结核分枝杆菌培养快速烟酸测定法的研究. 社区医学杂志, 2008, 6(9): 19-20.
［32］Singh P, Wesley C, Jadaun GP, et al．Comparative evaluation of Lowenstein-Jensen proportion method, BacT/ALERT 3D system, and enzymatic pyrazinamidase assay for pyrazinamide susceptibility testing of Mycobacterium tuberculosis. J Clin Microbiol，2007, 45(1): 76-80．
［33］Subramanyam B, Sivaramakrishnan G, Dusthackeer A,et al. Phage lysin to control the overgrowth of normal flora in processed sputum samples for the rapid and sensitive detection of Mycobacterium tuberculosis by luciferase reporter phage assay. BMC Infect Dis,2013,13:44.
［34］Yamazaki T, Sato N, Yamashita K, et al. Antimicrobial susceptibility testing for Mycobacterium tuberculosis by the bioluminescence assay of mycobacterial ATP using filamentous cell treatment. Rinsho Byori，2000,48(2)：167-173.
［35］Froman S,Will Dw,Bogen E.Bacteriophage active against virulent Mycobacterium tuberculosis. I.Isolation and activity.Am J Public Health Nations Health,1954,44(10):1326-1333.
［36］Chauca JA, Palomino JC, Guerra H. Evaluation of rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis by a mycobacteriophage D29-based assay. J Med Microbiol, 2007,56(Pt3):360-364.
［37］李同心,崔振玲,汪小黎，等.噬菌体生物扩增法检测结核分枝杆菌阿米卡星耐药性方法的建立及应用评价.中国防痨杂志,2011,33(3):158-162.
［38］Stella EJ, de la Iglesia AI, Morbidoni HR,et al. Comparison of the performance of two mycobacteriophage D29-based protocols for fluoroquinolone susceptibility testing in Mycobacterium tuberculosis. J Microbiol Methods, 2009, 79(3): 371-373.
［39］Moore AV, Kirk SM, Callister SM,et al. Safe determination of susceptibility of Mycobacterium tuberculosis to antimycobacterial agents by flow cytometry. J Clin Microbiol,1999,37(3):379-483.

[1]	Chinese Antituberculosis Association, Schools and Children Branch of the Chinese Antituberculosis Association, Editorial Board of Chinese Journal of Antituberculosis . Expert consensus of clinical application of the recombinant Mycobacterium tuberculosis fusion protein (EC) [J]. Chinese Journal of Antituberculosis, 2020, 42(8): 761-768.
[2]	YANG Lei, WEI Fen, ZHANG Kai, QIU Jing-jing, WANG Ying-ying, DU Wei-xin, LU Jin-biao, TAO Li-feng, PU Jiang. Stability and efficacy study of recombinant Mycobacterium tuberculosis fusion protein (EC) [J]. Chinese Journal of Antituberculosis, 2020, 42(8): 799-806.
[3]	ZHANG Kai, TAO Li-feng, WEI Fen, DU Wei-xin, QIU Jing-jing, CHEN Wei, CHEN Bao-wen, ZHU Yin-meng, CHENG Xing, SU Cheng, ZHONG Zai-xin, LU Jin-biao, PU Jiang. Immune characteristics and preclinical animal safety studies of recombinant Mycobacterium tuberculosis fusion protein(EC) [J]. Chinese Journal of Antituberculosis, 2020, 42(8): 807-813.
[4]	ZHANG Kai, SHEN Xiao-bing, TAO Li-feng, WEI Fen, CHEN Bao-wen, QIU Jing-jing, CHEN Wei, LU Jin-biao, ZHU Yin-meng, CHENG Xing, ZHONG Zai-xin, ZHAO Ai-Hua, PU Jiang. Establishment of quality standards for recombinant Mycobacterium tuberculosis fusion protein [J]. Chinese Journal of Antituberculosis, 2020, 42(8): 814-820.
[5]	DU Wei-xin, WEI Fen, LU Jin-biao, ZHAO Ai-hua, PU Jiang, WANG Guo-zhi, XU Miao. Preliminary establishment of national reference of the freeze-dried recombinant Mycobacterium tuberculosis allergen for the bulk potency evaluation [J]. Chinese Journal of Antituberculosis, 2020, 42(8): 826-831.
[6]	QU Meng-jin, LIANG Zheng-min, WANG Yuan-zhi, ZHOU Xiang-mei. Research progress of carbohydrate metabolism of Mycobacterium tuberculosis [J]. Chinese Journal of Antituberculosis, 2020, 42(8): 874-879.
[7]	SUN Qing, HUANG Hai-rong, WANG Gui-rong. In vitro activities and drug resistance mechanisms of bedaquiline, clofazimine and delamanid against common pathogenic non-tuberculous mycobacteria [J]. Chinese Journal of Antituberculosis, 2020, 42(8): 880-884.
[8]	SONG Yi-meng, LI Yuan-chun, HE Wen-cong, HE Ping, BAO Jing-jing, LIU Chun-fa, LIU Dong-xin, WANG Xin-yang, ZHAO Yan-lin, LI Yan-ming. Cost analysis of fluorescence PCR probe melting curve technique for the detection of Mycobacterium tuberculosis resistance in China [J]. Chinese Journal of Antituberculosis, 2020, 42(7): 712-717.
[9]	BAO Xun-di, JIANG Yue, LIANG Suo, CHENG Hong-yan, XIA Guang-xiu, WANG Chao, YE Qian, WANG Shu, WANG Qing. Analysis of the clinical isolation rate, population distribution and drug resistance of non-tuberculous mycobacteria in Anhui [J]. Chinese Journal of Antituberculosis, 2020, 42(7): 718-724.
[10]	ZHANG Jie, REN Yi-xuan, PAN Li-ping, ZHANG Zong-de. Application of whole genome sequencing in research of Mycobacterium tuberculosis [J]. Chinese Journal of Antituberculosis, 2020, 42(7): 737-740.
[11]	MA Jin-bao, REN Fei, ZENG Ling-cheng, CHEN Ming-wei. Analysis of drug susceptibility of 680 patients with multidrug-resistant tuberculosis in Xi’an City from 2015 to 2019 [J]. Chinese Journal of Antituberculosis, 2020, 42(6): 609-613.
[12]	QIN Zhong-hua, JING Ye, DU Yan-qing, SONG Xiao-mei, ZHANG Li-xia. Spectrum and drug resistance analysis of 339 strains of nontuberculous mycobacterium isolated from clinical practice [J]. Chinese Journal of Antituberculosis, 2020, 42(6): 630-633.
[13]	ZHANG Jing, CHEN Xi, WANG Bin, FU Lei, LU Yu, CHEN Xiao-you. Establishment of modified propidium monoazide (PMAxx)-quantitative PCR assay and its application for identification of antituberculosis drug activity [J]. Chinese Journal of Antituberculosis, 2020, 42(5): 472-480.
[14]	LI Xin-na, SHEN Xin-xin, WANG Rui-bai, DUAN Su-xia, ZHANG Rui-qing, WANG Rui-huan, BAI Xue-ding, FAN Guo-hao, WANG Jin-rong, GAO Yuan, CHEN Zi-wei, MA Xue-jun. Application value of probe-directed recombinase amplification assay in detecting MTB rpoB gene mutation [J]. Chinese Journal of Antituberculosis, 2020, 42(5): 481-488.
[15]	ZHANG Xing,WANG Feng-ming,LYU Xu-feng,HUA Tian-qi,ZHANG Xue-jun,JIANG Jing-yi,DING Chen-li,ZHU Wei,XIA Guo-dong,JI Jun-min,ZHAO Fei. Analysis of influencing factors of Mycobacterium tuberculosis infection in HIV/AIDS patients [J]. Chinese Journal of Antituberculosis, 2020, 42(4): 360-365.