Abstract: Objective  To analyze drug sensitivity of non-tuberculosis mycobacteria (NTM) in Guangzhou, to provide reliable scientific basis for the clinical treatment.  Methods  14 095 mycobacterial isolates were identified by traditional mycobacterial species identification method. 1819 NTM isolates were determined their sensitivity to 13 first-line and second-line anti-tuberculosis drugs (including INH, RFP, S, EMB, Am, Clr, Lfx, Mfx, CIP, Depasic, Rfb, Pto, Cm).  Results  Of 1819 NTM isolates, the INH and depasic resistant rates reached 96.15%（1749/1819）and 95.41%（1248/1308）, other drug-resistant rates were 76.25% (1387/1819) (S), 73.94% (817/1105) (Pto), 72.94% (954/1308) (Lfx),72.51% (1319/1819) (RFP),70.42% (1281/1819) (EMB),65.29% (854/1308) (Mfx),60.13% (543/903) (CIP),56.87% (236/415) (Rfb)and 50.50% (102/202) (Cm), respectively. While Clr- and Am-sensitive rates were 88.17%（1163/1319）and 67.73%（892/1317）, respectively.   Conclusion  NTM showed natural resistance to the first-line anti-tuberculosis drugs. The macrolide Clr and Amino sugar glycoside have stronger bacteriostatic effect on NTM. NTM isolates need perform the drug sensitivity testing.