Abstract: Objective To explore the clinical application value of particle colorimetric  technique in rapid detection of Mycobacterium tuberculosis （Mtb） resistance to pyrazinamide (PZA).  Methods 102 strains of Mtb resistance to PZA was measured by particle colorimetric technique, and was compared with the results of BACTEC MGIT-960 Mycobacterium detection system by American BD Corporation. Results Through particle colorimetric technique, in 102 strains of Mtb, 70 of them were PZA sensitive, 32 were PZA resistant. While under the method of BACTEC MGIT-960, 69 of them were PZA sensitive, 33 were PZA resistant. The two methods are consistent in 67 sensitive strains and 30 resistant strains. If we treat the susceptibility results of BACTEC MGIT-960 as the standard, for particle colorimetric technique, the sensitivity of PZA resistance was 90.9% (30/33), specificity was 97.1% (67/69), positive predictive value of 93.8% (30/32), negative predictive value of 95.7% (67/70), accuracy was 95.1% (97/102).  Conclusion Particle colorimetric technique is rapid and convenient in Mtb-PZA-resistance measurement. This method does not need any special apparatus and instrument and has high clinical application value.

Key words: Mycobacterium tuberculosis, Indicators and reagents, Pyrazinamide, Microbial sensitivity tests