Efficiency of fluorescence microscopy with light emitting diodes in detecting Mycobacterium tuberculosis

Abstract

Abstract: Objective To evaluate the efficiency of fluorescence microscopy with light emitting diodes (LED-FM) in screening Mycobacterium tuberculosis. Methods Sputum samples of 232 suspected patients were collected and there were totally 696 samples including 3 samples from each patient. All samples were detected by Ziehl-Neelsen (Z-N) stain microscopy and LED-FM respectively. Results The positive rates of LED-FM and Z-N were 37.9%（88/232）and 26.7%（62/232） with significant difference (χ²=6.66, P＜0.01). Conclusion LED-FM in detecting Mycobacterium tuberculosis is superior to the conventional method.

Key words: Mycobacterium tuberculosis, Microscopy,fluorescence, Staining and labeling

CHEN Yan-mei, QIAN Ming, JIANG Yong, CHEN Tao, LI Hai-cheng, ZHONG Qiu. Efficiency of fluorescence microscopy with light emitting diodes in detecting Mycobacterium tuberculosis[J]. Chinese Journal of Antituberculosis, 2011, 33(9): 585-587.

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Efficiency of fluorescence microscopy with light emitting diodes in detecting Mycobacterium tuberculosis

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