Abstract: Abstract：Objective  To evaluate the value of twelve antigens from  M. tuberculosis on the serodiagnosis of tuberculosis (TB), and to develop more effective diagnostic reagents for tuberculosis. Method  470 human sera were detected the antibodies against twelve M. tuberculosis antigens by ELISA, which sensitivities and specificities were evaluated. Results  The sensitivities of antibody detected by single antigen were range from 13.3%（28/210） to 40.5%(85/210), and the specificities were range from 96.4%(185/192) to 99.5%(191/192). The sensitivity and specificity of antibodies detected by multiple antigens can reach 65.2%(137/210) and 92.2%(177/192), respectively. Conclusion  The multiple antigens can increase the sensitivity and specificity of anti-TB antibody detection, and it may be valuable in the diagnosis of TB.

Key words: Tuberculosis/diagnosis, Mycobacterium tuberculosis , Antigens,bacterial, Serologic tests