A study of pulmonary surfactant associated protein A、D for diagnosis in pulmonary tuberculosis

Abstract

Abstract: Objective To study the expression of pulmonary surfactant associated protein A、D(SP-A、SP-D) and their correlation in patients with pulmonary tuberculosis Methods The expression of SP-A、SP-D of sputum and serum in 50 patients with smear positive pulmonary tuberculosis, 51 patients with smear negtive pulmonary tuberculosis,30 patients with COPD and 85 healthy volunteers were detected by ELISA. Results The expression of SP-A、SP-D of sputum in patients with smear positive pulmonary tuberculosis were correlated with the amount ofmycobacterium tuberculosisin sputum from these patients.The expression of SP-A、SP-D of serum in patients with pulmonary tuberculosis were significant higher than that of others（P<0.05）.But the expression of SP-D of sputum in patients with pulmonary tuberculosis were significant lower than that of others（P<0.01）. Conclusions The expression of SP-A、SP-D in patients are correlated with pulmonary tuberculosis. They may be an adjuvant marker to diagnose pulmonary tuberculosis.

Key words: tuberculosis/diagnosis, pulmonary surfactant-associated protein A, pulmonary surfactant-associated protein D, SP-A,SP-D

Hu Hua,Ju Yunfei,Xu Yong,Meng Fanmin,Chi Jingyu,Yang Yan,Li Xuezheng. A study of pulmonary surfactant associated protein A、D for diagnosis in pulmonary tuberculosis[J]. Chinese Journal of Antituberculosis, 2011, 33(6): 366-368.

References

［1］Ferguson JS,Voelker DR,McCormack FX,Schlesinger LS. Surfactant protein D binds toMycobacterium tuberculosisbacilli and lipoarabinomannan via carbohydrate-lectin interactions resulting in reduced phagocytosis of the bacteria by macrophages［J］. J Immunol,1999,163(1):312-321.
［2］马玙,朱莉贞,潘毓萱. 结核病［M］. 北京:人民卫生出版社,2006：103-226.
［3］中华医学会呼吸病学分会慢性阻塞性肺疾病学组. 慢性阻塞性肺疾病诊治指南（2007年修订版）［J］.中华结核和呼吸杂志, 2007,30(1):8-17.
［4］ Creuwels LA,van Golde LM,Haagsman HP.The pulmonary surfactant system: biochemical and clinical aspects［J］. Lung,1997,175(1):1-39.
［5］ Kuroki Y, Takahashi M, Nishitani C. Pulmonary collectins in innate immunity of the lung［J］. Cell Microbiol, 2007,9(8):1871-1879.
［6］ Wright JR. Immunoregulatory functions of surfactant proteins［J］. Nat Rev Immunol, 2005,5:58-68.
［7］ Jobe A,Ikegami M,Jacobs H, Jones S, Conaway D. Permeability of premature lamb lungs to protein and the effect of surfactant on that permeability［J］. J Appl Physiol,1983,55: 169-176.
［8］Hickman-Davis JM,Fang FC,Nathan C,Shepherd VL,Voelker DR,Wright JR. Lung surfactant and reactive oxygen-nitrogen species: antimicrobial activity and host-pathogen interactions［J］.Am J Physiol lung Cell Mol Physiol,2001,281(3):Ｌ517-523.

［9］Samten B, Townsend JC, Sever-Chroneos Z, Pasquinelli V, Barnes PF, Chroneos ZC.An antibody against the surfactant protein A (SP-A)-binding domain of the SP-A receptor inhibits T cell-mediated immune responses toMycobacterium tuberculosis ［J］. J Leukoc Biol, 2008,84(1):115-123.
［10］Ferguson JS,Voelker DR,Ufnar JA,Dawson AJ,Schlesinger LS. Surfactant protein D inhibition of human macrophage uptake ofMycobacterium tuberculosisis independent of bacterial agglutination［J］. J Immunol, 2002, 168(3):1309-1314.
［11］ Gold JA,Hoshino Y, Tanaka N, Rom WN, Raju B, Condos R, Weiden MD. Surfactant protein A modulates the inflammarory response in macrophages during tuberculosis［J］. Infect Immun,2004,72(2):645-650.

[1]	HE Hua, ZHOU Yu-jie, WEN Gao-yan, WANG Xin-ning, DING Chang, ZENG Yi. The diagnosis and differential diagnosis value of interferon gamma induced protein 10 (IP-10) in tuberculosis [J]. Chinese Journal of Antituberculosis, 2015, 37(4): 393-396.
[2]	SONG Yuan-yuan, ZHENG Hui-wen, ZHAO Yan-lin. The development of TB laboratory diagnostics in China [J]. Chinese Journal of Antituberculosis, 2014, 36(9): 764-768.
[3]	ZHANG Li-qun, LIU Yi-dian, ZHU You-sheng, TANG Shen-jie. Review and prospect for tuberculosis diagnosis and treatment in China [J]. Chinese Journal of Antituberculosis, 2014, 36(9): 769-773.
[4]	Clinic Society of Chinese Antituberculosis Association. Annual report on clinical diagnosis and treatment progress of tuberculosis (2013) (Part 1 clinical diagnosis) [J]. Chinese Journal of Antituberculosis, 2014, 36(8): 714-740.
[5]	CAI Xing-shan, MA Pin-yun, ZHANG Yuan-liang, TAN Yao-ju. Evaluation of the simultaneous amplification and testing for diagnosis of Mycobacterium tuberculosis [J]. Chinese Journal of Antituberculosis, 2014, 36(6): 458-461.
[6]	LI Hui, TAN Yao-ju, LI Hong-min, MA Xiao-guang, XING Jin, HAO Bao-lin, ZHAO Yan-lin. Evaluation of GeneXpert Mtb/RIF assay for diagnosis of tuberculosis and rifampin-resistant tuberculosis [J]. Chinese Journal of Antituberculosis, 2014, 36(6): 472-476.
[7]	ZHAO Mei-fen, LIU Ying-xia, PENG Zhong-tian, CHEN Chuan-tie, LIU Jing, LIN Yi-min, CHEN Jian-bo. Study on diagnostic value for active tuberculosis by detecting Mycobacterium tuberculosis specific antibodies in the peripheral blood [J]. Chinese Journal of Antituberculosis, 2014, 36(5): 356-361.
[8]	JIANG Jun. Quality control and quality assurance of T-SPOT.TB [J]. Chinese Journal of Antituberculosis, 2014, 36(5): 380-384.
[9]	ZHANG Deng-cai, LIU Bin, ZHANG Li-hua, SHI Min, SU Qin-jun, YANG Yan-li, QIAN Zhen, HA Ying-di. The value of acid-fast staining in pathological diagnosis of tuberculosis [J]. Chinese Journal of Antituberculosis, 2014, 36(4): 274-278.
[10]	GE Yan-ping，XIAO He-ping. The comparison of interferon-gamma release assay and tuberculin skin test on auxiliary detection of extrapulmonary tuberculosis [J]. Chinese Journal of Antituberculosis, 2014, 36(3): 194-197.
[11]	MA Yan, DU Jian, LI Liang, LIU Yu-hong. Systematic review on impact of different medical institutions mix model on tuberculosis case detection [J]. Chinese Journal of Antituberculosis, 2014, 36(12): 1055-1061.
[12]	Chinese Antituberculosis Association of Clinic Society. Annual report on clinical diagnosis and treatment progress of tuberculosis(2012) (Part 1 clinical diagnosis) [J]. Chinese Journal of Antituberculosis, 2013, 35(6): 405-426.
[13]	XU Miao, PU Jiang, YANG Lei, LU Jin-biao, TAO Li-feng, ZHONG Zai-xin, CHEN Bao-wen, DU Wei-xin, WANG Guo-zhi. Risk assessment and control for clinical trial of new tuberculosis intradermal diagnostic reagents [J]. Chinese Journal of Antituberculosis, 2013, 35(6): 460-462.
[14]	WANG Li-hong, FU Xiu-hua, ZHANG Gui-zhi, LI Guo-hua, GU Yan, GAO Jun-zhen. The value of T-SPOT.TB assay in the diagnosis of tuberculosis [J]. Chinese Journal of Antituberculosis, 2013, 35(12): 992-996.
[15]	LIU Jia-wen, KANG Li-jun, WENG Sheng-feng, ZHU Xin-ying. Diagnostic value of quantitative diagnostic kit for Mycobacterium tuberculosis-IFN-γ release assay [J]. Chinese Journal of Antituberculosis, 2011, 33(9): 600-603.