Evaluation of loop-mediated isothermal amplification (LAMP) in rapid detection of Mycobacterium tuberculosis

Abstract

Abstract: Objective To evaluate the value of loop-mediated isothermal amplification (LAMP) in rapid detection of Mycobacterium tuberculosis . Methods A set of four LAMP primers were designed for recognizing six distinct regions on the target DNA (gyrB). The DNAs ofM. tuberculosisstrains from Beijing Chest Hospital were extracted,amplified at 65°C for 60 min, and their reaction Results were observed by simple naked eye. Results A total of 79 clinical isolates and standard strains were studied. The sensitivity and specificity of LAMP was 100% and 92%, respectively.  Conclusions LAMP is a rapid and reliable alternative method for diagnosingM. tuberculosisinfection.

Key words: M. tuberculosis, nucleic acid amplification techniques

Dai Guangming,Cao Yicheng,Chen Xun,Du Zhengping,Huang Shuhai,Pang Yu,Zhou Yang,Huang Hairong，Zhao Yanlin. Evaluation of loop-mediated isothermal amplification (LAMP) in rapid detection of Mycobacterium tuberculosis[J]. Chinese Journal of Antituberculosis, 2011, 33(1): 47-51.

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