Abstract: Objective To evaluate the effect of Global Fund Project in treatment and management of pulmonary tuberculosis (PTB) patients among floating population and provide relevant scientific evidence for tuberculosis control strategy development.   Methods Treatment and management information of PTB patients of floating population before and after project implementation were collected and analyzed.   Results Before and after project implementation, the system management rate, cure rate of new smear positive, cure rate of retreatment smear positive, treatment completion rate of smear negative increased from 63.6% to 84.7%, 64.0% to 89.4%, 56.3% to 78.9%, 64.6% to 82.9% respectively. Except retreatment cure rate, all rates are statistically improved.   Conclusion The Global Fund Project play a positive role in tuberculosis case treatment and management, and lay a good foundation for tuberculosis control strategy development in floating population.