Identification of Beijing genotype of Mycobacterium tuberculosis using multiplex PCR

Abstract

Abstract: Objective To develop a new method to identify the Beijing genotype strains ofMycobacterium tuberculosis(MTB), and to analyze the spread and characteristics of Beijing family among the population.  Methods 112 MTB isolates were identified as Beijing genotype and non-Beijing genotype by Spoligotyping and multiplex PCR.  Results Of 112 MTB strains, 107 isolates were determined as Beijing genotype. Multiplex PCR could identify all of the Beijing stains with 100% sensitivity and specificity. Conclusion s Compared with Spoligotyping, multiplex PCR could rapidly differentiate Beijing and non-Beijing stains, it is simple, cost-effective and suitable for monitoring the spread of Beijing family strains on a large scale.

Key words: Mycobacterium tuberculosis, oligonucleotides, Beijing genotype, polymerase chain reaction

CLC Number:

R450

Zhao Defu,Xie Tong,Ju Hanfang,Mu Cheng,Zhao Hui. Identification of Beijing genotype of Mycobacterium tuberculosis using multiplex PCR[J]. Chinese Journal of Antituberculosis, 2010, 32(6): 21-23.

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