effect of the improved minimum inhibitory concentration test on detection of drug-resistantMycobacterium tuberculo

Abstract

Abstract: Objective To develop an improved minimum inhibitory concentration (IMIC) test to detect drug-resistantMycobacterium tuberculosisclinical isolates.  Methods 135 clinical isolates detected the drug susceptibility using traditional proportional method were retested and verified their sensitivity to isoniazid (INH), rifampin (RFP), ethambutol (EMB), streptomycin (SM) and ofloxacin (OFLX) by IMIC test.  Results In the tubes with same drug concentration, 68.89% of 135 clinical isolates with 1×10⁷/ml bacterium number inoculated got the same Results as those with 1×10⁵/ml bacterium number inoculated, and 31.1% was inconsistent. 14.8% (20/135) of the isolates (including 1 RFP-resistant, 3 INH-resistant, 3 OFLX-resistant，5 EMB-resistant and 8 SM-resistant strains) were judged the Results from negative to positive. Conclusion s The improved minimum inhibitory concentration test could increase positive detectable rate of drug-resistantMycobacterium tuberculosis , and find more potential drug-resistant TB patients.

Key words: Mycobacterium tuberculos, microbial sensitivity tests

CLC Number:

R446.5

Zhang Jianyuan,Zhao Yanlin,Sun Zhaogang,Jiang Guanglu,Li Yan,Wang Sumin,Liu Yi,Nie Lihui,Gao Tiejie,Zhang Zhiguo,Ren Weicong,Li Chuanyou. effect of the improved minimum inhibitory concentration test on detection of drug-resistantMycobacterium tuberculo[J]. Chinese Journal of Antituberculosis, 2010, 32(6): 16-20.

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