Abstract: Objective To investigate the antibiotic application related to anti-TB second-line drug (SLD)in general hospitals and TB specialist hospital in Shenzhen, and to provide a reliable basis for rational use of antibiotics and their scientific management. Methods Three general hospitals，including 245 inpatient cases from respiratory disease, digestive and urologic departments，and 96 cases fromTB special hospital were investigated in order to understand their application of antibiotic related to anti-Tuberculosis SLD. Cases were selected sequentiallyby their inpatient record number and investigated by recording the types and time period of applied antibiotic related to anti-tuberculosis second line drugs（SLD）.   Results Only 15.9% of all inpatient cases had drug susceptibility test, and 59.6% of cases had ever applied antibiotic related to anti-tuberculosis second line drugs. The majority applied Fluoroquinolone, accounting for 49.8%, followed by Macrolides, accounting for 9%. Fluoroquinolone classes are mostly focused on the use of levofloxacin and moxifloxacin, while the macrolides are more concentrated in azithromycin and clarithromycin; Department of Respiratory Medicine used more often, accounting for 75.1%. Among 96 cases of PTB from TB special hospital, 65 cases of them are diagnosed as PTB without other bacterial infection, and 52% of them combined use of second line anti-TB drugs; 31 cases of PTB complicating with other bacterial infection all applied SDL, and 81% of them had no DST Results . The majority applied Fluoroquinolone, accounting for 89%, and focused on Levofloxacin. Aminoglycoside antibiotics is seldom used.  Conclusion s The PTB treatment is not standard in TB special hospital. Use of antibiotic was more of empiric-based. Fluoroquinolone and Macrolides were excessively used both in general and TB special hospitals in Shenzhen.

Key words: antibiotics,antitubercular, fluoroquinolones, macrolides, hospitals,special, hospitals,general