Co-expression of protein of M. tuberculosis with molecular chaperone

Abstract

Abstract: Objective To elucidate the effect of chaperones co-expression on increasing the expression of protein from M. tuberculosis encoding genes, and on enhancing biology activity of the protein expressed. MethodsCo-expressing the plasmids Rv3790::pET16b and Rv3791::pET16b which harbor protein encoding gene Rv3790 and Rv3791 of Mycobacterium tuberculosis with chaperones plasmid pkJE7 which can express 3 chaperones,DnaK,DnaJ,GrpE at the same time in E.coli.BL21(DE3).The yield of the expression was then checked by SDS-PAGE and Western Bloting. The biology activity of the expressed candidate proteins was analyzed by related activity assay. The expression system, which expressed the candidate genes alone in E.coli. BL21(DE3), was used as control. ResultsCompared with the control, the co-expression system could produce more soluble protein, less inclusion body and less degradation of protein. When same amount of protein was used in the activity assay, the protein from the chaperone co-expression system had higher activity than that from the non-chaperone co-expression system. ConclusionCo-expression with chaperone could increase protein expression and protein activity of M. tuberculosis.

Key words: protein expression, molecular chaperone, co-expression

Huang Hairong¹, Dong Xu, Zhang Zongde, Zhao Yanlin, Jiang Guanglu, Li Qiang. Co-expression of protein of M. tuberculosis with molecular chaperone[J]. Chinese Journal of Antituberculosis, 2009, 31(2): 76-79.

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