Abstract: ObjectiveTo evaluate the application value of tuberculosis antibody and enzyme-linked immuno-spot assay used for Clinical diagnosis of TB.MethodsThe two Methods were used individually and in combination respectively to 83 active tuberculosis patients (43 Mycobacterial tuberculosis positive patients, 40 Mycobacterial tuberculosis negative patients), 50 nontuberculosis in control group.ResultsMycobacterium positive rate were 79％detected with 38kD-IgG and 93.0％ with T SPOT-TB． The combined detection positive rate was 97.7％． The TB culture negative patient’s Mycobacterium positive rate were both 72.5％ tested with the two single method． And the combined tested positive rate was 92.5％． To Non-TB control group, the specificity rate of the 2 Methods were 70.0％ and 94.0％ respectively. Combined tested specificity rate was 66％． ConclusionsTo TB positive patients， T Spot-TB is more sensitivity than 38kD-IgG (93％)． But there was no significantly statistical difference (χ²=3.49， P>0.05) between them． T SPOT-TB test specificity was significantly higher than 38kD-IgG test (χ²=9.76，P<0.01)． Joint detection of the two Methods can significantly improve the tuberculosis positive rate in culture negative patients． Compared with the single detection method， the difference was statistically significant (χ²=5.54，P<0.05)， As a result， the joint testing for TB-negative patients have more practical value.

Key words: tuberculosis/diagnosis, antibodies,bacterial, immunoglobulin G, immunoenzyme techniques