Abstract: Objective To evaluate the application value of protein-chip in detecting anti-tuberculosis(TB) antibodies in the clinical samples. Methods The serum samples from 117 patients with TB,and 103 patients with non-tuberculosis lung diseases and healthy volunteers were detected anti-TB antibodies using protein-chip.The sputum samples from 117 patients with TB were also observed acid-fast bacilli by smear and microscopy as control. Results The positive rate of antiTB antibodies were 100%(26/26),49.6%(45/91) in smear-positive and smear-negative patients with pulmonary tuberculosis by using protein chip,respectively.The negative rate of anti-TB antibodies was 98.1%(101/103) in the patients with non-tuberculosis lung diseases and healthy volunteers by detection of protein chip. Conclusion The anti-TB antibody protein-chip is a simple,rapid,high-through,sensitive,specific,low cost,and effective method for the diagnosis of TB,especially for the smear-negative TB.

Key words: Pulmonary tuberculosis/diagnosis, Sputum, Protein-chip