Diagnostic values and clinical significance of detecting soluble FasL of pleural effusions in differentiating tuberculous and malignant pleural effusions

Abstract

Abstract: Objective To explore the diagnostic values and clinical significance of detecting soluble FasL of pleural effusions in differentiating tuberculous and malignant pleural effusions.Methods Using enzyme-linked immunosorbent assay(ELISA) method to detect the soluble FasL in 43 tuberculous pleural effusions and 32 malignant pleural effusions.Results The mean of soluble FasL of tuberculous pleural effussions(13.56±5.38ng/ml)was markedly higher than that of malignant pleural effusions(5.72±2.59ng/ml)(P＜0.001).If 10ng/ml of soluble FasL was regarded as cutoff value,the sensitivity,the specificity and accordance of diagnosing tuberculous pleural effusions were 81.4%,81.3% and 81.4%,respectively.Conclusion Soluble FasL might be a effective laboratory marks of differentiating tuberculous and malignant pleural effusions.

Key words: Soluble FasL, Malignant pleural effusions, Tuberculous pleural effusions, ELISA

Liu Yufeng¹,Che Wenhua,Shi Yugui,et al.. Diagnostic values and clinical significance of detecting soluble FasL of pleural effusions in differentiating tuberculous and malignant pleural effusions[J]. Chinese Journal of Antituberculosis, 2007, 29(4): 324-327.

References

1 朱元珏，陈文彬.呼吸病学[J].北京：人民卫生出版社,2003:1011
2 Jones EY, Stuart Di, Walker NP. Structure of turner necrosis facter, 1989,383:225-228
3 Nagata S, Golstein P. The Fas deash facter [J]. Science, 1995, 267,(5203)：1449
4 SCHULZE OK,Ferrari D,Los M,et al. Apoptoeis signaling by dealh[J].Eur J Biochem, 1998,254 (3) ：439-459
5 Mustafa,T,Bjune T,G; Jonsson,R,et al. Increased expression of fas lig-and in human tubercuiosis and leprosy lesions: a potential novel mechanism of immune evasion in mycobacterial infection, ScanD L b immunol.2001,54(6)：630
6 Tanaka M, Suda T ,Takahaahi T, et al . Expression of the functionl soluble form of human Fas Ligand in activated lymphocytes[J]. EMBL J, 1995,14:1129
7 Suda TtTANAKA M,Miwa K, et al. Apoptosis of mouse native T cells in-duced by recombinant soluble Fas Ligand and activation induced resistance to Fas Ligan[J] ,J Immunol, 1996,157:3918
8 李嫣红，乐晓画，周伯平，等.结核性淋巴结炎组织中的检测[J].中华结核和呼吸杂志,2000,23(12):737
9 Oddo, M ；Renno , T, Attinger, A, et al . Fas ligand infected human macrophages reduces the viability of intracellular Mycobacterium tubercu-losis[J]. J immund 1998,160(11):5448-5450,181(4)：1506-9
10 Mustafa,T; Bjune T, G; Jonsson, R, et al. Increased expression of Fas ligand in human tuberculosis and leprosy leaions: a potential novel mechansism of Immune evasion in mycobacterial infection[J]. Scand J b,Immunol.2001,54(6):630-639
11 Schroter M, Pell J, hahne M, et al. Fas dependent tissue turnover is implicated in turner cell cleaance[J] .Oncogene. 2000,19(14)：1794
12 Wu,S.H;Chu, J,J.Chang, CD,et al.increased soluble Fas ligand con-centration in tuberculous pleural effusions [J] , J Formos Med Assoc.2001,100(l):32-34

[1]	Wang Yingnian , Liu jiahong , Sun Rongli, et al.. The clinical diagnostic value of detection of P-selectin, IL-1α, sFas, sFasL, AM in differentiating tuberculous pleural effusions from malignant pleural effusions [J]. Chinese Journal of Antituberculosis, 2007, 29(1): 8-11.
[2]	Liu Jiahong, Wang Yingnian, Gong Xitao, et al.. The mensuration and clinical values of the soluble Fas in tuberculosis patitents [J]. Chinese Journal of Antituberculosis, 2005, 27(4): 224-226.
[3]	XIANG Yan-gen,SHI Guo-min.. PCR microplate hybridization-ELISA for identification of tubercle bacillus DNA [J]. Chinese Journal of Antituberculosis, 2003, 25(4): 264-266.
[4]	ZHAO Yi hong,HUANG Run tang,CAO Song,et al.. The clinical observation of the present duration of anti TB-IgG in the cured tuberculosis patients [J]. Chinese Journal of Antituberculosis, 2001, 23(6): 379-381.
[5]	ZHANG Xiao-gang,ZHUANG Yu-hui,HE Xiu-yun,et al.. Evaluation of the recombinant 38kD and 16kD antigen of Mycobacterium Tuberculosis for serodiagnostic test [J]. Chinese Journal of Antituberculosis, 2001, 23(5): 281-283.
[6]	DU Qiong,YANG Zheng-lin,LUO Tao,et al.. Extrapulmonary tuberculosis diagnosed by microcell plate hybridization assay [J]. Chinese Journal of Antituberculosis, 2000, 22(4): 211-213.