Abstract: Objective To investigate the possible role of HLA in incidence of pulmonary tuberculosis patients in Guangdong in China,we study the promoter structure of HLA susceptive gene DRB1*040101-44. Methods The promoter sequence of HLA susceptive gene DRBl*040101-44 was searched in nucleotide database in NCBI(http://ncbi.nlm.nih.gov/),and according to the conservational nucleotide sequence specific primers was designed to amplify the promoter structure of HLA susceptive gene DRBl*040101-44 by PCR,then the production was reclaimed for PCR direct sequencing;finally the promoter sequences of HLA susceptive gene DRBl*040101-44 in pulmonary tuberculosis patients was compared with normal people and with those sequence published in NCBI. Results The group of pulmonary tuberculosis patients had the same promoter sequences of HLA susceptive gene DRBl*040101-44 as control group.But it was different with those published in nucleotide database in network,and had 6 point mutations in Y component box: T in position-166 was replaced by A,C in-150 by G,T in-137 by G,C in-121 by G,T in-98 by C,A in-73 by G.There were no mutations in nucleotide sites from position-93 to-88 in TATA box,from position-130 to-125 in CAAT box and from position-189 to-179 in X box. Conclusion The promoter structure of HLA susceptive gene DRBl*040101-44 had polymorphism,its nucleotide mutation happened in Y-box,but the polymorphism had no association with development of pulmonary tuberculosis in Guangdong in China.

Key words: Pulmonary tuberculosis, Sequence,promoter, HLA-DRBl