Abstract: Objective To explore the in-vivo culturing technique of mycobacteria from special hand infections in laboratory,and to provide the direct evidence for clinical diagnosis.Method To review Acid-Fast Stain,mycobacterial culture,identification by chemistry and gene sequesing of 28 cases of hand special chronic infections.Results Only 2 cases were positive in Acid-Fast Stain,10 cases showed positive by mycobacterial culture,and 8 M.marinum,1 M.fortuitum,1 M.avium.Conclusion Mycabacterium were the major factor of hand special chronic infections,and of all non-tuberculous mycobacterium(NTM),M.marinum was the most important one which should be paid more attention when culturing.It is necessarily to culture them at both 35℃ and 30℃.

Key words: NTM, Gene identification