A research on effect of anti-germ for Multiresistant M.tuberculosis with antituberculous drugs in combination

Abstract

Abstract: Objective To explore the role of antituberculous drugs in common use and in combination against Multidrug-resistant Mycobacterium tuberculosis with another antituberculous drugs.Methods To determine MIC and MICs each single drug and in combination to different Multidrug-resistant M.tuberculosis strains,according to FIC formula,count out the synergetic activitives.Results FIC of in combination of INH,RFP,EMB,CAM and INH,RFP,PZA,OFLX are less than 0 5.Conculusion In vitro,constitution of INH,RFP,EMB,CAM and INH,RFP,PZA,OFLX have been confirmed that have synergetic effect to multidrug-resistant M.tuberculosis.

Key words: M.tuberculosis, Drug resistant, Fractional inhibition concentration (FIC)

MA Hai-zhan,CHEN Zheng.. A research on effect of anti-germ for Multiresistant M.tuberculosis with antituberculous drugs in combination[J]. Chinese Journal of Antituberculosis, 2000, 22(3): 157-160.

References

[1] Person ML, Jereb JA, Frieden TR, et al. Nosocomical transmission of multidrug-resistant mycobacterium tuberculosis. A risk to patients and health care workers [J].Ann Intern Med, 1992,117：191-196
[2] Gobld M, Iseman MD, Madsen LA, et al. Treatment of 171 patients with pulmonary tuberculosis resistant to isoniazid and rifampin [J]. N Engl J Med.1993,328:527-532.
[3] WOH Global TB Programme. WHO Report on the Tuberculosis Epidemic 1996[R].Geneva.1996.
[4] Leonid H. Qualitative and quantitative drug-susceptibility tests mycobacteriology [J]. Am Rev Respir Dis,1998,137：1217-1222.
[5] Dickinson J M. Aber VR, Mitchiaon A. Bactericial activity of strepto-mycin, isoniazid, rifampin, ethambutol. and pyrazinamide alone and in combination against Mycobacterium tuberculosis [J]. Am Rev Respir DIS, 1997,116:627-635.
[6] Nicola A. Carione Acocella, Anna Maria Cuffini，Martina Fcxno-piz-zoglio. Killing of macrophage-ingested Mycobacteria by rifampicin 9 pyrazinamide,and pyrazinoic acid alone and in combination [J]. Am Rev Respir Dis, 1985,132：1274-1277.
[7] Stephen JC，Jon RB. Synapstic activities of clarithrranycin and antitu-berculos drugs against multidrug-resistant Mycobacterium tuberculosis [J]. Antimicrobial Agents and Chemotherapy,1995,1542-1545.
[8] 罗永艾,王宠林，黄中臣，等.复治肺结核病人痰菌耐药不均一性研究[J]，中国防痨杂志,1991,13(4)：166-168.
[9] Alfred JC,Mary HM. Inhibition of tubercle bacilli in cultured human macrophages by chloroquine used alone and in combination with strep-tomycin, isoniazid, pyrazinamide, and two metabolites of vitamin D₃ [J] .Antimicrobial Agents ang Chemotherapy.1990,2217-2222.
[10] 宋文虎，肖成志,宋礼章，主编.结核病学进展[M].北京：光明日报出版社，1995,95-113.
[11] Alferd JC,Max SF,Mary HM. L-25(OH)₂ Vitamin D₃ synergizes with pyrazinamide to kill tubercle bacilli in cultured human macrophages. [J].Am Rev Respir Dis,1989,139:549-552.
[12] Leonid H, Pamela L. Pyrazinmide steering activity in vitro against semidormant Mycobacterium tuberculosis bacterial populations [J]. Am Rev Respir Dis,1992,145:1223-1225.
[13] Alfred JC, Elise JR. Comparative abilities of various metabolites of vitamin D to protect human macrophages against tubecler bacilli [J].Journal of Leukocyte Biology,47：545-550

[1]	JIANG Ming-xia, CHAO Xiu-zhen, WANG Zhao-fen, MA Bin-zhong, MA Yong-cheng, WANG Rong, WANG Chao-cai, LI Er-chen, WEI Yu-jia, LI Yong-xue, ZHANG Qi. Drug resistance analysis of 154 Mycobacterium tuberculosis strains in southern Qinghai [J]. Chinese Journal of Antituberculosis, 2020, 42(12): 1289-1293.
[2]	FENG Ying, MA Jin-bao, YANG Han, YANG Yunan-li, REN Fei. Analysis of the drug sensitivity test results of the mycobacterium isolates from cerebrospinal fluid of 93 patients with tuberculous meningitis [J]. Chinese Journal of Antituberculosis, 2020, 42(11): 1177-1182.
[3]	YING Ruo-yan, HUANG Xiao-chen, WANG Jie, LIU Yi-dian, SHA Wei, YANG Hua. Clinical analysis of drug sensitivity tests of various drug-resistant MTB isolates against different combinations of anti-tuberculosis drugs in vitro [J]. Chinese Journal of Antituberculosis, 2020, 42(11): 1183-1182.
[4]	Jian YANG,Tian-hua ZHANG,Xiao-ping XIAN,Yan LI,Rui WANG. Analysis of drug resistance of clinical isolates of smear positive pulmonary tuberculosis patients in Shaanxi Province from 2017 to 2018 [J]. Chinese Journal of Antituberculosis, 2019, 41(6): 616-623.
[5]	SHI Lian,REN Yi-jie,XI Ying,SHI Lei,GUO Chen,GUAN Jian. Detection value analysis of three laboratory techniques for diagnosing tuberculosis and drug resistance in smear-positive patients [J]. Chinese Journal of Antituberculosis, 2019, 41(11): 1173-1178.
[6]	Yan HU,Jie LIU,Jing SHEN,Da-mian ZHU,Xin FENG,Lin CHEN,Jian ZHAN,Xi-chao OU,Yang ZHOU,Yan-lin ZHAO. Prevalence and molecular characterization of multidrug-resistant Mycobacterium tuberculosis isolates resistant to fluoroquinolones in Chongqing [J]. Chinese Journal of Antituberculosis, 2018, 40(10): 1060-1065.
[7]	WU Yuan, HU Pin-Pin, WANG Yi-Wei, LI Xiu-Quan, MAO Xu-Hu, ZHONG Min. Polymorphism Analysis of gene iniB that encodes the hypothetical protein Rv0341 of M. tuberculosis by RFLP [J]. Chinese Journal of Antituberculosis, 2010, 32(11): 756-759.
[8]	Gao Tiejie,Zhang Zhiguo,Du Chunying,et al.. Rapid detection of drug susceptibility testing in Mycobacterium tuberculosis by nitrate reductase assay [J]. Chinese Journal of Antituberculosis, 2008, 30(4): 338-341.
[9]	Li Hongmin,Liang Jianqin,Wang Wei,et al.. The research of the relationship between Km gene mutation and the drug-resistance of M.tuberculosis [J]. Chinese Journal of Antituberculosis, 2008, 30(1): 37-39.
[10]	Chen Xiaorong¹,Zhang Xia1,Shi Xudong1,et al.. Feasibility study on rapid identification of Mycobacterium tuberculosis complex and non-tuberculosis mycobacterium using BACTEC MGIT 960 system [J]. Chinese Journal of Antituberculosis, 2008, 30(1): 40-42.
[11]	Yuan Baodong¹,Wag Weihua,Duan Qionghong,et al. Evaluating the quality of life of patients with multi-drug resistant pulmonary tuberculosis [J]. Chinese Journal of Antituberculosis, 2006, 28(3): 139-142.
[12]	Pan Zhifen¹, Yu Shouchang ,Zhang Ruying,et al.. The impact of air-conditions on transmission of M tuberculosis in hospital [J]. Chinese Journal of Antituberculosis, 2005, 27(3): 151-153.
[13]	Huang Xuerui,Gao Weiwei,Bu Jianling,et al.. Analysis of therapeutic effect on the individualized regimens for 69 patients with multi-drug resistant pulmonary tuberculosis [J]. Chinese Journal of Antituberculosis, 2004, 26(5): 257-260.
[14]	Ai Yiming,Huang Ling,Li Guoming,et al.. Surveillance of drug resistant tuberculosis in Huangshi of China [J]. Chinese Journal of Antituberculosis, 2004, 26(4): 230-232.
[15]	Gao Chunrong,Yuan Dongyun,Xu Lijiang.. The short-term treatment effect of regimen which contains the Amikacin Sulfate and the Levofloxacin for multi-drug resistant pulmonary tuberculosis cases (MDR-TB) [J]. Chinese Journal of Antituberculosis, 2004, 26(3): 165-167.