Abstract: Objective To explore the reformation on the m ethod of drug sensitivity test for Mycobacteriu m tu berculosis . Method 50 M . T B was separated from the clinic by the 7 H12 Bliquid mediu m used for B A C T E C. Afterdiluted at different density ,directly vaccinated on the reform ed L珔o wenstein Jensen medium . Com paring with thedrug sensitivity results of B A C T E C,the reform ed drug sensitivity method could be valued . Result 1 . The nu mberof M . T Bin 1 ml 7 H12 Bliquid m ediu m( G I500 ～900)is equal to that of 1 m g wet weight of M . T B(106 ～7) .2 . Thebacteria liquid draw n fro m 7 H12 B liquid medium was directly vaccinated on the traditional L珔o wenstein Jensenmedium . With the drug sensitivity result co mparing with that of B A C T E C,there is no significant difference( P>0.05) . Conclusion The m ethod of B A C T E Cdrug sensitivity testis usually used for only 4 kinds of anti tuberculo sis drugs . The reform ed method could be used for more and remedy the defect of B A C T E C. Com paring with themethod of L珔o wenstein Jensen drug sensitivity test ,the time the new method used was short of 13 to 15 days .

Key words: BACTEC, Drug susceptibility test