Development and challenges of Mycobacterium tuberculosis cfDNA in the diagnosis of tuberculosis

doi:10.19982/j.issn.1000-6621.20220228

Abstract

Abstract:

In order to achieve the goal of “End Tuberculosis by 2035”, it is urgent to develop new detection methods. Mycobacterium tuberculosis cfDNA (MTB-cfDNA) can indicate the presence of pathogens and is an attractive biomarker for tuberculosis (TB) diagnosis and treatment monitoring. Easily accessible MTB-cfDNA in urine and/or blood can be used to detect both pulmonary and extrapulmonary TB in any age group. The diagnostic performance of MTB-cfDNA in fluid samples such as pleural effusion, ascites and cerebrospinal fluid is higher than that of genomic DNA assays such as GeneXpert MTB/RIF. Research on cfDNA as a TB biomarker is still in its infancy, larger and clinical studies including more comprehensive patient types are needed to evaluate the utility of cfDNA in TB diagnosis. With further optimization of detection methods, cfDNA testing may improve TB diagnosis and become a “game changer”.

Key words: Mycobacterium tuberculosis, Genes,bacterial, Diagnosis, Review literature as topic

CLC Number:

R378.91+1

Peng Lijun, Fang Tingting, Cai Long. Development and challenges of Mycobacterium tuberculosis cfDNA in the diagnosis of tuberculosis[J]. Chinese Journal of Antituberculosis, 2022, 44(10): 1091-1095. doi: 10.19982/j.issn.1000-6621.20220228

Figures/Tables 1

第一作者	样本类型	确诊方法	靶标	检测方法	产物大小 (bp)	涂片阳性 (%)	敏感度 (%)	特异度 (%)
Ushio^[28]	血浆	培养	IS6110 gyrB	dPCR	137	100	65(21/33) 29(10/33)	93(18/19) 100(19/19)
Yan $g [29] a$	全血	综合诊断	IS6110	dPCR qPCR	83	-	100(28/28) 50(14/28)	100(28/28) 100(28/28)
Yan $g [29] b$	全血	综合诊断	IS6110	dPCR qPCR	83	-	100(28/28) 54(15/28)	100(28/28) 100(28/28)
Click^[30]	血浆	培养、Xpert	IS6110	qPCR	106	100	45(18/40)	2/3
韩冰^[31]	血浆	涂片、培养	IS6110	qPCR	-	-	79.70(94/118)	95(58/61)
Lyu^[34]	血浆	涂片、培养	IS6110 IS1081	dPCR	71 71	-	40.6(63/155) 27.1(42/155)	93.4(99/106) 93.4(99/106)
Pan^[33]	血浆	培养、病理和分子	IS6110	qPCR	71	45.8	54.2(13/24)	96.5(55/57)
Park^[32]	血浆	培养、分子	IS6110	PCR	-	31.6	80.0(32/40)^c 47.5(19/40)^d	78.1(32/41) 85.4(35/41)
Cannas^[39]	尿液	涂片、培养	IS6110	半巢式PCR	67/129	95	79(34/43)	100(23/23)
Labugger^[43]	尿液	培养	IS6110	PCR	38	60	64(7/11)	8/8
Patel^[42]	尿液	培养	DR区	PCR	38	33	43(75/175)	89(210/237)
Oreskovic^[41]	尿液	Xpert	IS6110	PCR	40	38.8	83.7(41/49)	100(24/24)
Che^[20]	胸腔积液	综合诊断	IS6110	qPCR	-	-	75(45/60)	100(18/18)
寿娟^[21]	胸腔积液	综合诊断	IS6110	qPCR	-	12.0	63(65/103)	100(33/33)
Yang^[22]	胸腔积液	综合诊断	IS6110	qPCR	-	-	69.23(153/221)	100(65/65)
Sharma^[23]	腹腔积液	综合诊断	devR	qPCR	139	-	70.9(22/31)	97.1(33/34)
Haldar^[24]	脑脊液	培养	IS6110 devR	PCR qPCR	200 144	-	85.2(69/81) 87.6(71/81)	92(72/86) 92(79/86)
Haldar^[25]	脑脊液	培养	devR	qPCR	144	-	100(29/29)	96(125/130)
Shao^[26]	脑脊液	综合诊断	IS6110	qPCR	75	-	53.3(32/60)	100(24/24)
Li^[27]	脑脊液	综合诊断	IS6110	qPCR	-	-	56.5(26/46)	100(22/22)

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