Study on the application effect of ultrasonic dispersion technique in the reprocessing of contaminated mycobacterium liquid cultures

doi:10.19982/j.issn.1000-6621.20220217

Abstract

Abstract:

Objective: Study on the application effect of ultrasonic dispersion technique in the reprocessing of mycobacterium liquid culture contaminated specimens. Methods: Sputum samples of 4490 suspected tuberculosis patients and 795 confirmed tuberculosis patients admitted to the Outpatient and Inpatient Departments of Shandong Public Health Clinical Center were subjected to initial liquid culture, and the positive rate, contamination rate and reporting time of contamination were observed. Among them, there were 259 cases of culture contamination. The liquid culture of every contaminated specimen was thoroughly mixed and divided into two samples, one was reprocessed directly, and the other was reprocessed by ultrasound dispersion method. The positive rate and contamination rate in groups using the two methods were observed, and the detection rate of Mycobacterium tuberculosis complex (MTBC) and nontuberculous mycobacteria (NTM) were compared. Results: The positive rate of primary culture of 5285 sputum specimens was 17.7% (935/5285), and the contamination rate was 4.9% (259/5285). Among the 259 contaminated samples, 157 cases (60.6%) reported contamination time within 1-3 days, 100 cases (38.6%) within 4-10 days, 2 cases (0.8%) within 11-20 days. The positive rates of direct group and ultrasonic group were 7.3% (19/259) and 13.9% (36/259), respectively; contamination rates were 26.6% (69/259) and 21.2% (55/259); MTBC detection rates were 4.2% (11/259) and 5.0% (13/259); NTM detection rates were 3.1% (8/259) and 8.9% (23/259), respectively. Comparing the culture positive rate between the two groups, the ultrasound group was statistically higher than the direct group (χ²=5.879, P=0.015). The detection rate of NTM in the ultrasound group was statistically higher than that in the direct group too (χ²=7.720, P=0.005), while no statistical difference was observed for detection rate of MTBC between the two groups (χ²=0.175,P=0.676). Conclusion: The ultrasonic dispersion method can better detect mycobacteria and especially for NTM while controlling the contamination, indicating that it has great application potential in the reprocessing of mycobacterial liquid culture contaminated specimens.

Key words: Mycobacterium tuberculosis, Mycobacterium, Clinical laboratory techniques, Specimen handling

CLC Number:

R446

Tian Peng, Deng Yunfeng, Wang Junling, Ling Xiaojie, Zhang Zhenjin, Wang Lin. Study on the application effect of ultrasonic dispersion technique in the reprocessing of contaminated mycobacterium liquid cultures[J]. Chinese Journal of Antituberculosis, 2022, 44(11): 1162-1166. doi: 10.19982/j.issn.1000-6621.20220217

Figures/Tables 1

References 24

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