Abstract: Objective  To learn the awareness status of Beijing health care workers’ (HCWs) knowledge about tuberculosis(TB) infection control in TB facilities and general hospitals. Methods   A cross-sectional study was conducted among 1390 HCWs in 3 TB facilities and 4 hospitals in Beijing. Questionnaires were used to learn about the HCWs’ knowledge of tuberculosis infection control.   Results  HCWs have a good awareness of isolation and ventilation, the rate of correct cognition are 98.2%(1365/1390) and 98.1%(1363/1390) respectively in all HCWs; but the cognitive status about the knowledge of personal protection, especially about triage(72.0%(1001/1390)), disinfection(54.0%(751/1390)), are dissatisfied. There is a significant difference between the knowledge of four aspects of TB infection control among  doctors and nurses, that is,triage, disinfection, whether we should wear masks or not when contacting with suspected or confirmed TB patients and what type of masks we should choose when contacting with these patients. As for triage, 68.1%（320/470）doctors know correctly about it compared with 74.0%（681/920）nurse(χ²=5.374, P=0.020);As for disinfection, 46.0%(216/470)doctors know correctly about it compared with 58.2%(535/920) nurses(χ²=18.608, P<0.01);As for whether we should wear masks when contacting with suspected or confirmed TB patients,78.1%(367/470)doctors know correctly about it compared with 87.5%(805/920)nurses(χ²=20.050, P<0.01);As for the right type of masks to choose, 34.9%(164/470)doctors know correctly about it compared with 41.1%(378/920)nurses(χ²=5.053, P=0.025). Nurses have a higher correct response rate than doctors.  Conclusion  We should strengthen the knowledge and skills about TB infection control among the HCWs. Importance should be attached to the knowledge of triage, disinfection, the type of masks and the wearing condition in personal protection.