The molecular epidemiology of Mycobacterium tuberculosis in Beijing

Abstract

Abstract: Objective Typing of Mycobacterium tuberculosis strains and epidemiological studies in Beijing.Methods (1)To detect and analyze the restriction fragment length polymorphism (RFLP) of M.tuberculosis isolated from 128 new patients with pulmonary tuberculosis by using standardized method.(2)A sociodemographical and epidemiological questionnaire investigated all patients selected in this study.(3)The relationship of genotype of M.tuberculosis and their epidemiology were analyzed.Results (1)128 M.tuberculosis detected were belonged in 3 groups, according to their homology of IS6110 fingerprinting.The strains in A and B groups were called as “Beijing genotype strains” because these strains had high homology, and they had a high percentage (103/128,80.5%) in all strains detected.Strains in group C, called as “non-Beijing genotype strains”,had lower homology and only 19.5% in all strains detected.(2)The polymorphism of the strains isolated from the younger patients (<40) were different significantly compared with the strains isolated from those elder patients (>40).The selectivity predominance of “Beijing genotype” was not due to BCG vaccine widely.(3)The polymorphism of the strains detected were not correlated significantly to past TB contracted history of the patients investigated,living in urban or rural area of Beijing and the drug-resistance of the strains.Conclusion Fingerprinting analysis of M.tuberculosis can be a useful tool for the study of modern epidemiology of tuberculosis.

Key words: Mycobacterium,tuberculosis, DNA fingerprint, Tuberculosis epidemiology, Beijing

ZHANG Li-xing,DING Bei-chuan,TU De-hua,et al.. The molecular epidemiology of Mycobacterium tuberculosis in Beijing[J]. Chinese Journal of Antituberculosis, 2001, 23(3): 141-147.

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