Abstract:

Objective To evaluate the anti-tuberculosis activity of traditional Chinese medicine named Shengluwan in vitro and in mouse tuberculosis model.Methods The minimal inhibitory concentration (MIC) of Shengluwan against Mycobacterium tuberculosis standard strain H37Rv was determined by 7H9 liquid medium and 7H10 solid medium. A mouse tuberculosis acute model was set up through aerosol infection to SPF BALB/C mice using Mycobacterium tuberculosis standard strain H37Rv. On the 10th day of infection, drug treatments were begun with 3g/kg of Shengluwan or 25mg/kg of isoniazid, respectively. Ten mice in each group were orally administered by gavage for 3 weeks or 6 weeks treatments. At the end of treatment, the mice were sacrificed, weighed, sterilized under sterile operation and lungs CFU counted. The CFU between the groups were compared.Results The MIC of Shengluwan against H37Rv was 2.5mg/ml in 7H9 liquid medium, and less than 5mg/ml in 7H10 solid medium. All mice were tolerant during the period of infection and treatment. There were no death mouse. After administration of 3 weeks, the CFU of the lungs in Shengluwan group were (6.81±0.71)lg CFU, which was 0.39lg CFU lower than that of the control group. After 6 weeks treatment, the CFU of the whole lungs was (4.33±0.51)lg CFU in the Shengluwan group, which was 1.10lg CFU lower than the control group.Conclusion In the mice tuberculosis model, Shengluwan shows the anti-tuberculosis activity in mice. It is worth further study in the future.

Key words: Mycobacterium tuberculosis, Drugs, chinese herbal, Treatment outcome, Comparative study, Shenglu wan