Chinese Journal of Antituberculosis

Table of Content

10 April 2013, Volume 35 Issue 4

Risk factors for drug-resistant tuberculosis in China: analysis of the results of the national drug resistant tuberculosis baseline survey in 2007

WANG Sheng-fen, ZHAO Bing, SONG Yuan-yuan, ZHOU Yang, OU Xi-chao, LI Qiang, XIA Hui, PANG Yu, DUANMU Hong-jin, FU Yu, ZHAO Yan-lin

Chinese Journal of Antituberculosis. 2013, 35(4): 221-226.

Abstract ( 1795 )

PDF (790KB) ( 999 )

Save

References | Related Articles | Metrics

Objective The aim of our study was to identify risk factors associated with drug resistant tuberculosis（TB） and Multidrug-resistant TB （MDR-TB） in new and retreatment cases. Methods A national drug resistance TB baseline survey was carried out from April 2007 to December 2007, the survey with multistage cluster sampling, stratified by provinces, was conducted in 31 provinces, autonomous regions and municipalities directly under the central government. Seventy clusters representative of the tuberculosis prevention and control system of the country were randomly selected, eligible new diagnosed smear-positive tuberculosis patients including 51 new cases and 17 retreatment patients were recruited into each cluster during the survey. Relevant data of the patients were collected by physician through questionnaire. The patients were asked about the history of treatment through face-to-face interview; if possible, patients’ medical records were reviewed to validate the information. The accuracy of treatment history was confirmed by another physician through re-interviewing. Drug susceptibility testing to isoniazid, rifampicin, streptomycin, ethambutol, kanamycin and ofloxacin was performed on Lowenstein-Jensen (L-J)medium according to the proportion method. Results The multivariate analysis results showed that new cases who had TB treatment for less than one month（OR=1.6, 95% CI＝1.1－2.1）were significantly more likely to have drug resistant TB; while female gender （OR=1.4, 95% CI＝1.0－2.1）and TB treatment （OR=2.4, 95% CI＝1.5－3.7）were independent risk factors for the presence of MDR-TB in new cases. Retreatment patients who were female gender（OR=1.7, 95% CI＝1.1－2.7）, who had previously been treated for TB for more than 2 times and the last treatment was provided by TB hospital （OR=4.0, 95% CI＝1.2－14.0）were significantly more likely to have drug resistant-TB. While female gender（OR=2.3, 95% CI＝1.5－3.6）, live in area with later DOTS implementation (after 2000)（OR=1.7, 95% CI＝1.2－2.4）, previous TB treatment for more than 2 times and the last treatment was provided by medical facility other than TB hospital（OR=3.3, 95% CI＝2.1－5.2）, previous TB treatment for more than 2 times and the last treatment was provided by TB hospital（OR=13.0, 95%CI＝3.9－46.0）were independent risk factors for the presence of MDR-TB in retreatment patients. Conclusion New cases who have a history of TB drug treatment are at high risk for developing drug resistant TB and MDR-TB; while female gender and previous TB treatment for more than 2 times are independent risk factors for the development of drug resistant TB and MDR-TB in retreatment patients.

Psychological treatment and the effect on patients’ adherence of smear positive TB patients in floating population in Guangzhou

CHEN Xun-xun, ZHONG Qiu, ZHOU Lin, GAO Cui-nan, XU Zhuo-wei

Chinese Journal of Antituberculosis. 2013, 35(4): 227-230.

Abstract ( 1677 )

PDF (739KB) ( 663 )

Save

References | Related Articles | Metrics

Objective To explore the effect of psychological treatment for the smear positive TB patients in floating population in Guangzhou and its effect on patients’ treatment adherence. Methods Psychological states of the 640 new smear positive TB patients who were diagnosed and initiated treatment from September 2, 2008 to April 30, 2010 were evaluated by Symptom Checklist 90 (SCL-90) and Social Support Rating Scale (SSRS). One hundred and sixty-four cases with psychological obstacles were randomly divided into control group and intervention group with 82 cases each. 2H₃R₃Z₃E₃/4H₃R₃ was applied to both groups while comprehensive psychological intervention (mainly supporting treatment) was applied to the intervention group. The differences of psychological states and treatment compliance rates at the end of the second month between the two groups were compared. t test (measurement data) andχ² test (count data) were applied and the level of significance was set to P<0.05. Results The psychological obstacles of the 164 patients were mainly reflected in four factors (somatization，anxiety，obsession and depression). After intervention the scores of the factors in control group were 1.51±0.53, 1.50±0.46, 1.75±0.54 and 1.65±0.61 respectively. While those of the intervention group were 1.25±0.49, 1.27±0.44, 1.50±0.50 and 1.36±0.60, which were significantly lower than the domestic norms (1.37±0.48、1.39±0.43、1.62±0.52、1.50±0.59) (t=2.21, 2.46, 2.17 and 2.11, P<0.05) and the control group（t=3.27, 3.28, 3.08 and 3.09，P<0.01）. The scores of SSRS in the interventions (22.67±5.51, 8.75±3.15, 7.98±2.19 and 37.23±6.67) were higher than those in the controls (20.56±4.43, 6.93±2.21, 7.01±2.42 and 33.87±5.43), and the differences had statistical significance (t=2.70, 4.28, 2.69 and 3.53，P<0.01). The treatment compliance rate in the intervention group at the end of the second month’s treatment (96.3%，79/82） was significantly higher than that in the control group (82.9%，68/82)(χ²=7.94, P<0.01). Conclusion Psychological intervention in floating TB patients can effectively improve their treatment adherence.

Systematic review of latent tuberculosis infection and tuberculosis preventive therapy

LIU Er-yong, ZHOU Lin, CHENG Shi-ming

Chinese Journal of Antituberculosis. 2013, 35(4): 231-239.

Abstract ( 2809 )

PDF (748KB) ( 1777 )

Save

References | Related Articles | Metrics

Objective To describe and evaluate the progress of the latent tuberculosis infection and tuberculosis preventive therapy. Methods We made a comprehensive search for published literatures including 7 databases, websites of 3 health agencies and Google Scholar. Studies related to the diagnosis of latent tuberculosis infection and tuberculosis preventive therapy were identified by inclusion and exclusion criteria. Initially 573 papers were searched out, and 17 papers were included eventually. Of the 17 papers included in the analysis, 4 studies aimed to describe the status of latent tuberculosis infection. 2 were guidelines published by US CDC, 11 studies amid to evaluate the treatment regimen and effectiveness of preventive therapy. Results The Mycobacterium tuberculosis infection rate of the entire population of United States is about 4.2%, 14.2% for non-Aboriginal population of Columbia of Canada, 15.0% for the 8 provinces of Afghanistan, and our fourth epidemiological survey results showed this rate was 44.5% for all age group of China. (United States, Canada, Afghanistan use the tuberculin skin test induration diameter≥ 10 mm as standard of infection, and China use induration diameter≥ 6 mm as standard). Latent infection diagnosis is based on the comprehensive consideration of patients’ past history, the tuberculin skin test or interferon-γ release test results, chest radiological examinations, physical examination. The effect of different regimen of preventive treatment varied greatly, with the protection rate ranging from 0% to 61%, the treatment completion rate from 43% to 90%, the incidence of adverse drug responses mainly rash, hepatitis, and peripheral neuritis from 0% to 10%. Conclusion The current focus of TB control in China is to achieve the purpose of rapid and effective control of TB transmission through the detection and cure of infectious cases. However, with the development of China’s economy and the intensified TB case detection, tuberculosis preventive therapy will become an important part of TB control measures.

Analysis of the medical expenses and economic burden of pulmonary tuberculosis patients in three cities

WANG Qian，WANG Li-xia，LI Ren-zhong，RUAN Yun-zhou，CHEN Ming-ting，SUN Qiang

Chinese Journal of Antituberculosis. 2013, 35(4): 240-245.

Abstract ( 2165 )

PDF (692KB) ( 831 )

Save

References | Related Articles | Metrics

Objective To analyze the medical expenses and economic burden of pulmonary tuberculosis(TB) patients in Hohhot,Kaifeng and Lianyungang city, and to provide a basis for the future development of TB control policies and strategies. Methods We randomly sampled 100 cases of pulmonary TB patient from all the local patients who had ever treated in the TB specialist hospitals (including outpatient and inpatient) between April 30, 2008 and April 2009 in the three cities and had completed the treatment before the survey. In November-December 2009, we completed the site investigation of the total 100 cases of pulmonary TB patients, 32 cases from Hohhot of Inner Mongolia, 32 cases from Kaifeng City of Henan and 36 cases from Lianyungang City of Jiangsu respectively. The purpose of the survey is to understand the economic situation of patients’ family and the expenses during the treatment period, and analyze medical expenses and economic burden of patients. Results Among 100 cases of patient investigated in the three cities, the average monthly medical expenses accounted for 94.6% to 119.0% of household average monthly non-food expenditure. 84.4% (27/32), 90.6% (29/32) and 88.9% (32/36) of patients respectively in Hohhot, Kaifeng and Lianyungang had catastrophic health expenditure. Conclusion The medical expenses of pulmonary TB in three cities is high, and of burden the disease economic was heavy.

Evaluation of the implementing effect of TB control program during 2001—2010 in Xinjiang Uygur Autonomous Region

XUE Feng，WU Wei-dong，Yipar，TAI Xin-rong, YAO Xiao-xiong,LI Yue-hua，YANG Jin-min，GU Xiao-ming

Chinese Journal of Antituberculosis. 2013, 35(4): 246-250.

Abstract ( 1610 )

PDF (679KB) ( 441 )

Save

References | Related Articles | Metrics

Objective To evaluate the implementation effect of the 10 years TB control program from 2001 to 2010 in Xinjiang Uygur Autonomous Region, and to summarize the achievements and experiences, and provide recommendations for the sustainable development of TB control program in Xinjiang. Methods According to the final evaluation plan of national TB program (2001—2010), the evaluation forms and annual and quarterly surveillance data in 14 prefectures and 96 counties of Xinjiang from 2001—2010 were collected, and EpiData was used for data input and Excel for data analysis. Results In 10 years, the DOTS coverage rate reached 100%, the total TB control funding was 201.75 million Yuan RMB in Xinjiang. Cumulatively a total of 225 771 active pulmonary TB patients were detected, of which 133 315 were smear positive TB cases. 219 800 cases of active pulmonary tuberculosis were treated which included 127 200 cases of smear positive cases. The cure rate reached 93.37%(124 478/133 315). Conclusion Through the implementation of the 10 years TB control program, Xinjiang Uygur autonomous region has reached the goal of the high case detection rate and high cure rate, and achieved remarkable effect.

Factor affecting the publication delay of articles of Chinese Journal of Antituberculosis from 2006 to 2011

FAN Yong-de, XUE Ai-hua, ZHANG Xiao-jin, GUO Meng, YANG Ying

Chinese Journal of Antituberculosis. 2013, 35(4): 251-254.

Abstract ( 1194 )

PDF (679KB) ( 333 )

Save

References | Related Articles | Metrics

Objective To explore the influencing factors of delay in publication of articles (DPA) of Chinese Journal of Antituberculosis from 2006 to 2011, and to provide reference for magazine quality improvement and development. Methods The publication delay is defined as the time difference between the receipts of the manuscript to the publication date. According to the provisions promulgated by the Science and Technology Commission of the People’s Republic of China on the quality requirements and evaluation criteria of the top-five scientific journals, the DPA less than 280 days is defined as short delay, and more than 280 days as long delay. The DPA of a total of 708 original papers published between 2006 to 2011 in 54 issues were analyzed by SAS 9.1. Results The average DPA was 246.67 days and the coefficient of variation was 51.37%(126.71/246.67) from 2006 to 2011. Majorities were short delays accounting for 61.72%（437/708）, long delay accounted for 38.28%（271/708）. The multivariate logistic regression analysis showed that eastern region (eastern vs. middle and western β=0.71, s_x=0.18，OR=2.04，95%CI=1.44-2.87), funding support (yes vs. no β=0.68, s_x =0.19，OR=1.97，95%CI=1.36-2.87), public health paper (public health paper vs. basic research paper β=0.60,s_x=0.20，OR=1.83，95%CI=1.23-2.72; public health paper vs. clinical paper β=0.52, s_x=0.19，OR=1.68，95%CI=1.16-2.43) were factors significantly associated with the average DPA. Conclusion The DPA of Chinese Journal of Antituberculosis is affected by the region and content of the study, and whether with funding support.

Analysis of clinical characteristics and serum lipid level in pulmonary tuberculosis patients with diabetes mellitus

YAO Ying，WANG Jun，LIU Yu-feng，WANG Qiu-zhen，SUN Li-mei，JIANG Guo-feng，SUN Lin，MA Ai-guo

Chinese Journal of Antituberculosis. 2013, 35(4): 255-259.

Abstract ( 1663 )

PDF (705KB) ( 684 )

Save

References | Related Articles | Metrics

Objective The objective of this study was to find out the clinical characteristics and nutritional status of the pulmonary tuberculosis patients with diabetes mellitus (PTB-DM) and provide the targeted suggest for their clinical treatment and nutritional support. Methods One hundred and sixteen inpatients with pulmonary tuberculosis complicated with diabetes mellitus in Qingdao Chest Hospital from 2011.12 to 2012.8 were recruited as the PTB-DM group. And 136 pulmonary tuberculosis inpatients without diabetes mellitus of this hospital in the same period were selected as the PTB group. Between the two groups, compared the incidences of clinical symptoms, erythrocyte sedimentation rate (ESR) sped up, sputum smear positive and the nutrition status indicators including total cholesterol (TC), triglyceride (TG) and total lymphocyte count (TLC). Divided the PTB-DM group into well-controlled group（34 cases） and poor-controlled group（82 cases） according to their fasting blood glucose, and analysed the effect of glycemic control on clinical characteristics at the time of admission. Results The incidences of cough，expectoration，hemoptysis，fatigue，anorexia，ESR sped up and positive sputum smear in PTB-DM group were 97.4%（113/116），90.5%（105/116），31.0%（36/116），68.1%（79/116），39.7%（46/116），81.9%（95/116）and 78.4%（91/116）, while those of PTB group were 88.2%（120/136），80.9%（110/136），14.0%（19/136），55.1%（75/136），27.9%（38/136），66.9%（91/136） and 66.2%(90/136）respectively. These values in PTB-DM group were obviously higher than those in PTB group(χ²=7.565，4.639，10.684，4.422，3.866，7.272，4.659 respectively；all P<0.05).The incidences of expectoration，hemoptysis，fatigue and positive sputum smear in well-controlled group were 79.4%（27/34），17.6%（6/34），52.9%（18/34）and 64.7%（22/34），which were significantly lower than 95.1%（78/82），36.6%（30/82），74.4%（61/82）and 84.1%（69/82） in poor-controlled group（χ²= 6.911，4.028，5.090，5.373 respectively；all P<0.05）.The analyses of nutrition status showed that TC and TG in PTB-DM group were（4.39±1.17） mmol/L and （1.43±0.91） mmol/L while those in PTB group were （3.97±0.97） mmol/L and （1.01±0.51） mmol/L，PTB-DM group was higher than PTB group with significant statistic differences（t=-3.237，t′=-4.457 respectively；all P<0.05）；the TLC of PTB-DM group was（1.36±0.52）×10⁹/L，which was lower than（1.52±0.64）×10⁹/L in PTB group（t′=2.000，P<0.05）. Conclusion The PTB-DM patients’ condition are more serious and the TC and TG are higher. They should be given targeted diagnosis，treatment measures and diet guidances clinically.

The diagnosis of Mycobacterial coinfection among HIV/AIDS patients by combining liquid culture with strain identification technique

ZENG Zhao-ying，ZHAO Xiu-ying

Chinese Journal of Antituberculosis. 2013, 35(4): 260-263.

Abstract ( 1520 )

PDF (680KB) ( 373 )

Save

References | Related Articles | Metrics

Objective To evaluate the prevalence of HIV/Mycobacteria coinfection with liquid Mycobacteria culture and molecular technique in North China. Methods Fifty-three suspected HIV/AIDS patients were performed with liquid culture and strain identification of Mycobacteria from 2009 to 2011. The clinical manifestation，radiological imaging and relevant laboratory data from all patients with definite diagnosis of HIV/Mycobacteria coinfection were analyzed. Results Of 19 patients confirmed with HIV/Mycobacteria coinfection, Mycobacteriosis involved in pulmonary only in 11(57.9%),extra-pulmonary (brain and liver) in 2(10.5%), Mycobacteremia in 4(21.1%)，both pulmonary and mycobacteremia in 2(10.5%). Mycobacterium tuberculosis(16,84.2%) and Mycobacterium avium-intracellulare complex (3,15.7%)were isolated from the 19 patients. 15(78.9%) patients had a quite low CD4⁺T lymphocytes count less than 100 cells per microlitre in peripheral blood. Conclusion Combining liquid culture and molecular techniques is effective method to identify HIV/Mycobacteria coinfection and to be applied in the clinical practice.

Study on the relationship between the value of glycosytated hemoglobin and clinical manifestation in patients with diabetes combined with pulmonary tuberculosis

KONG Zhong-shun,MA Li-ping, ZHANG Li-qun,GAO Meng-qiu,MA Yu

Chinese Journal of Antituberculosis. 2013, 35(4): 264-267.

Abstract ( 1845 )

PDF (686KB) ( 315 )

Save

References | Related Articles | Metrics

Objective To investigate the relationship between the clinical manifestations and the value of glycosytated hemoglobin A1c (HbA1c) in patients with diabetes combined with pulmonary tuberculosis. Methods Three hundred and ninety-five cases with diabetes combined with pulmonary tuberculosis admitted in our hospital from the January 1, 2011 to December 31, 2011 were divided into three groups based on the HbA1c value detected the day after admission according to the standard from China Diabetes Prevention Guidelines. The three groups were as follows: group Ⅰ with HbA1c less than 6.5%(the well control group,40 cases), group Ⅱ with HbA1c ranged from 6.5% to 7.5%(the medium control group, 63 cases) and group Ⅲ with HbA1c more than 7.5%(the poor control group, 292 casas). The status of Mycobacterium tuberculosis in sputum and the characteristics of pulmonary lesions in CT imaging were compared anong the three groups. Statistics analysis was used the SPSS 19.0 software. Group t test was applied for measurement data and correction t test was applied if variance was heterogeneity. χ² test was applied for count data and linear trendχ² test was applied for linear trend of the three subgroups. Results The sputum positive rate was 50.0%(20/40) in group Ⅰ, 73.0%(46/63) in group Ⅱ and 90.4%(264/292) in group Ⅲ, respectively. The sputum positive rate in the group Ⅱ was much higher than that in the group Ⅰ and in the group Ⅱ (χ²=47.83,P<0.01). Morever, the sputum positive rate in the group Ⅲ was much higher than that in the group Ⅰ (χ²=5.63,P<0.05). The positive rate of sputum bacillus in the three groups presented an increasing trend(χ²_trend=47.46, P<0.01). The discovery rate of caseous lesions was 45.0%(18/40) in group Ⅰ, 58.7%(37/63)in group Ⅱ and 80.1%(234/292)in group Ⅲ, respectively. The discovery rate of caseous lesions in the group Ⅲ was much higher than that in the group Ⅰ and in the group Ⅱ (χ²=30.08,P<0.01).The discovery rate of caseous lesions in the three groups presented an increasing trend(χ²_trend=29.67, P<0.01). The discovery rate of worm-eaten-like cavities was 12.5%(5/40), 14.3%(9/63) and 28.4%(83/292)in the three groups, respectively. The discovery rate of worm-eaten-like cavities in the Ⅲ group was much higher than that in the group Ⅰ and in the group Ⅱ (χ²=9.08, P<0.05). The discovery rate of worm-eaten-like cavities in the three groups presented an increasing trend(χ²_trend=8.17, P<0.05).The discovery rate of caseous cavities was 25.0%(10/40)，27.0%(17/63) and 53.1%(155/292) in the three groups. The discovery rate of caseous cavities in the group Ⅲ was much higher than that in the group Ⅰ and in the group Ⅱ (χ²=22.16, P<0.01).The discovery rate of caseous cavities in the three subgroups presented an increasing trend(χ²_trend=19.57, P<0.05). Conclusion The relationship between the value of glycosytated hemoglobin and the clinical features is close in of the paitents with diabetes combined with pulmonary tuberculosis.

Study on the relationship between the value of glycosytated hemoglobin and clinical manifestation in patients with diabetes combined with pulmonary tuberculosis

KONG Zhong-shun,MA Li-ping, ZHANG Li-qun,GAO Meng-qiu,MA Yu

Chinese Journal of Antituberculosis. 2013, 35(4): 264-267.

Abstract ( 1001 )

PDF (686KB) ( 217 )

Save

References | Related Articles | Metrics

Clinical analysis on coexistence of pulmonary tuberculosis and lung cancer in 156 patients

LI Hua，LIANG Bo-wen，BU Jian-ling，LI Qi，CHEN Xiao-you

Chinese Journal of Antituberculosis. 2013, 35(4): 268-272.

Abstract ( 2043 )

PDF (688KB) ( 738 )

Save

References | Related Articles | Metrics

Objective To investigate the clinical characteristics and CT features of patients with pulmonary tuberculosis complicated with lung cancer and to distinguish these patients early. Methods We analyzed retrospectively the data, such as demography, clinical characteristics, relationship of lung cancer lesions and tuberculosis focus, confirmed time, pathological type of lung cancer，confirmed diagnosis methods, from 156 pulmonary tuberculosis patients complicated with lung cancer admitted in our hospital from Jan. 2000 to Dec. 2011. Results Pulmonary tuberculosis accompanied by lung cancer was more common in the aged male patients. Blood-stained sputum was mainly clinical characteristic in these patients. CT scan showed that most nodular lesions localized in upper lobe. Some lesions were presented as mass. The patients with lesions localized the same lobe of ipsilateral lung were 19 (12.2%). The lesions of pulmonary tuberculosis and lung cancer localized in different lobe among 72 cases(46.2%). In 65 cases, some lesions of pulmonary tuberculosis and lung cancer were overlapped partially. There were 67 cases diagnosed as lung cancer after pulmonary tuberculosis confirmed and 6 cases diagnosed as pulmonary tuberculosis after lung cancer confirmed. 83 cases were diagnosed as pulmonary tuberculosis and lung cancer no more than two months. The mean time of confirmed diagnosis was from 11.4 months to 150 months. The major pathological type was non small cell lung cancer (NSCLC). Productive cough and fiberoptic bronchoscopic brushing were the primary way to examine the tumor cells. Conclusion The patients with pulmonary tuberculosis complicated with lung cancer presented some special clinical characteristics and CT features. Diagnosis earlier is contributed to treat synchronously in time and to improve prognosis.

Investigation on the ability of Mycobacterium solid culture in four county (district) tuberculosis dispensaries

OU Xi-chao, LIN Bai-feng, HU Pei-lei, XIA Hui, ZHAO Yan-lin

Chinese Journal of Antituberculosis. 2013, 35(4): 273-275.

Abstract ( 1714 )

PDF (664KB) ( 359 )

Save

References | Related Articles | Metrics

Objective To evaluate the feasibility of Mycobacterium solid culture in county (district) tuberculosis (TB) dispensaries. Methods 2059 doubtful patients with pulmonary TB from Xiangtan, Yueyang, Beilin and Lanxi county were continuously recruited from May 2010 to February 2011. The sputum samples from patients recruited were collected and performed smear examination and solid culture. The smear-positive and culture-negative rate and culture pollution rate were calculated each month. Results (1) The smear-positive rate and culture-positive rate in all patients recruited were 18.3% (376/2059) and 26.6% (547/2059)，respectively. They had statistically significant difference (χ²=129.2, P<0.01) (2) The smear-positive and culture-negative rate in Beilin was 16.5% (23/139), higher than normal level. The rate of culture pollution in Xiangtan and Yueyang were 5.6% (157/2800) and 9.7%(252/2590)，respectively, both higher than normal level. The smear-positive and culture-ne-gative rates and culture pollution rates in other counties (district) were in normal range. Conclusion Four laborato-ries in county (district) TB dispensaries have the primary ability on performing Mycobacterium solid culture.

Spoligotyping and analyses of Mycobacterium tuberculosis isolates and “Beijing family” strains from Inner Mongolia, China

YU Qin, SU Yun-kai, LV Bing, MA Yan, LIAN Lu-lu, YANG Xiao-min, DONG Hai-yan, LIU Yao, ZHAO Xiu-qin, WU Yi-mou, WAN Kang-lin

Chinese Journal of Antituberculosis. 2013, 35(4): 276-281.

Abstract ( 2038 )

PDF (905KB) ( 428 )

Save

References | Related Articles | Metrics

Objective To study the genotypic constitution of M. tuberculosis isolates from Inner Mongolia, and the distribution characteristics of “Beijing family” strains in this area. Methods 372 M. tuberculosis clinical strains isolated from confirmed patients with tuberculosis in 2011, including the patients’epidemiological and clinical information, were collected from Institute for Tuberculosis Control and Prevention, Inner Mongolia and were genotyped by traditional 43 space Spoligotyping. The results of the DNA fingerprinting of these 372 strains were analyzed with BioNumerics 5.0 software, and compared with SpolDB 4.0 database. In addition, NTF and LSP were used to explore the characteristics of the “Beijing family” strains. Among these strains, 282 were from the patients of Han, 84 were from the patients of Mongolian, the rest including 4 strains, 1 strain and 1 strain were from Hui, Uygur and Manchu respectively. The relativity between the susceptibility of the Beijing genotype and the two major nationalities was analyzed. The statistical analyses were performed by the software of the SPSS 13.0, and the chi-square test was used to determine the susceptibility of the Beijing genotype in the two major nationalities (Mongolian and Han). Results 372 strains were divided into 48 genotypes by spoligotyping referring to SpolDB 4.0 database. According to the database, 24 genotypes were new types. “Beijing family” genotype was the most prevalent genotype (85.48%, 318/372), followed by the T family (4.84%, 18/372), H family (0.81%, 3/372), MANU family (0.27%, 1/372) and Latin American and Mediterranean family (0.27%, 1/372). Among these strains of the Han patients, 87.94%(248/282) were “Beijing family”, and 12.06% (34/282) were non-“Beijing family”. Meanwhile, there were 79.76%(67/84) “Beijing family” strains and 20.24% (17/84) non-“Beijing family” strains in the Mongolian. Finally, the chi-square test between susceptibility of the “Beijing family” genotype and the two major natio-nalities (Mongolian and Han) showed that there was no statistical significance (χ²=3.612, P=0.057). Conclusion There are genotypic diversities of M. tuberculosis clinical strains, and “Beijing family” genotype is the most prevalent family in Inner Mongolia. We did not find any relevance between the “Beijing family” genotype and the major nationalities.

The research progression of tuberculosis vaccine on antigen85 complex

Chinese Journal of Antituberculosis. 2013, 35(4): 282-285.

Abstract ( 3040 )

PDF (689KB) ( 564 )

Save

References | Related Articles | Metrics

Nowadays,the prevalence and incidence of tuberculosis are on the rise in the whole world.However,the maximum protection of the only used tuberculosis vaccine-Bacilli Calmette-Guérin is limited.Therefore,it is very urgent to research and develop a new effective vaccine.Ag85 cantigen 85 complex has received a great attention in the field of new vaccine against tuberculosis because it can enhance experimental animals’immunity.This article introduced some achievements about Ag85 complex vaccines.The results showed that researchers have demonstrated that the protection of a few vaccines exceed that of traditional BCG. As a priming vaccine,it is safety and effective for newborns, children and youngsters. As a booster regimen to adults,it can enhance the anti-tuberculosis ability. Moreover, the vaccine combined with chemotherapy can improve the immunity and curative effect.But it needs more clinical trical evidence to demonstrate wether if it could replace traditional BCG.