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Chinese Journal of Antituberculosis ›› 2022, Vol. 44 ›› Issue (5): 455-459.doi: 10.19982/j.issn.1000-6621.20210710

• Original Articles • Previous Articles     Next Articles

Preliminary study on serum pharmacochemistry of Niubeixiaohe extract

DUAN Li-yao1,2, LING Yan-bo1, LIANG Yan1, SHI Ying-chang1, WANG Lan1, ZHAO Guan-ren3, LIU Jun4, ZHENG Yue1(), WU Xue-qiong1()   

  1. 1Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Tuberculosis Research Institute, Senior Department of Tuberculosis, the 8th Medical Center of Chinese PLA General Hospital, Beijing 100091, China
    2Department of Pharmacy, Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine of Hebei Province, Cangzhou 061001, China
    3Department of Pharmacy, the 8th Medical Center of Chinese PLA General Hospital, Beijing 100091, China
    4Guangdong Qifang Pharmaceutical Co., Ltd., Guangzhou 510075, China
  • Received:2021-12-18 Online:2022-05-10 Published:2022-05-04
  • Contact: ZHENG Yue,WU Xue-qiong E-mail:zhengy309@126.com;xueqiongwu@139.com
  • Supported by:
    G20 Engineering Support and Guarantee Special Project of Beijing Municipal Science and Technology Commission(Z171100001717010);Beijing “Ten Diseases and Ten Medicines” Research and Development Special Project(Z141100002214002)

Abstract:

Objective: Preliminary research on serum pharmacochemistry was carried out on the extract of anti-tuberculosis Chinese medicine Niubeixiaohe, and the material basis of Niubeixiaohe’s efficacy was discussed by analyzing the blood components. Methods: From April 2018 to June 2019, the standard solutions of Radix Platycodonis, Rhizoma Bletillae,Herba Houttuyniae, Fructus Arctii and the sample of Niubeixiaohe were prepared at the Tuberculosis Research Institute, the Eighth Medical Center of Chinese PLA General Hospital. 55 mice were randomly divided into 11 groups with 5 mice each group: Group 1 to 5 named Niubeixiaohe extract group were given Niubeixiaohe extract 1.25 mg·g-1·d-1; Group 6 to 10 named arctiin group were given arctiin 0.3 mg·g-1·d-1; Group 11 was the blank control group, which was given distilled water. The mice were gavaged continuously for 7 days. Blood was collected from the retroorbital venous plexus of mice at 0.5, 1, 2, 4, and 6 hours after the last administration to prepare the serum solution. The retention time of each substance in the chromatogram of the standard of traditional Chinese medicine, Niubeixiaohe extract and the serum sample solution of mice in each group after administration was analyzed by HPLC. The constituents absorbed into the blood of Niubeixiaohe extract were preliminarily identified with the reference substance. Results: The overlapping chromatograms of Niubeixiaohe extract and each medicinal standard showed that the substance corresponding to the chromatographic peak at the retention time of 7.1 min was derived from Radix Platycodonis, and the substance corresponding to the chromatographic peak at the retention time of 13.5 min, 15.5 min and 21.2 min was derived from Rhizoma Bletillae, and the substance corresponding to the chromatographic peak at the retention time of 16.3 min was derived from Fructus Arctii, which was further identified as arctiin compound. Compared with the blank control serum, the serum samples after the administration of Niubeixiaohe extract and arctiin was found the absorption peaks in 17.5-22.5 min. Conclusion: HPLC can be used as a quality control method for the extraction of Radix Platycodonis, Rhizoma Bletillae, Herba Houttuyniae, and Fructus Arctii in Niubeixiaohe. The detected constituents absorbed into the blood of Niubeixiaohe extract may be arctiin metabolites, and the trace amount of other pharmacodynamic components into the blood was not detected.

Key words: Traditional Chinese medicine compound, Niubeixiaohe, Serum pharmacochemistry, High performance liquid chromatography, Antituberculosis agents

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