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中国防痨杂志 ›› 2021, Vol. 43 ›› Issue (1): 36-41.doi: 10.3969/j.issn.1000-6621.2021.01.008

• 论著 • 上一篇    下一篇

糖尿病与糖尿病并发肺结核患者的血浆代谢组学对比研究

王美杰, 李雨泽, 肖瑶, 吴明歧, 侯绍英()   

  1. 150081 哈尔滨医科大学公共卫生学院营养与食品卫生学教研室(王美杰、肖瑶、吴明歧、侯绍英);黑龙江省医院临床营养科(李雨泽)
  • 收稿日期:2020-05-07 出版日期:2021-01-10 发布日期:2021-01-12
  • 通信作者: 侯绍英 E-mail:hsy3982@163.com
  • 基金资助:
    国家自然科学基金面上项目(81872615);黑龙江省自然科学基金青年项目(QC2018092);黑龙江省省属高等学校基本科研业务费基础研究项目(2018-KYYWF-0439)

A comparative study of plasma metabolomics between diabetes and diabetes complicated with pulmonary tuberculosis

WANG Mei-jie, LI Yu-ze, XIAO Yao, WU Ming-qi, HOU Shao-ying()   

  1. Nutrition and Food Hygiene,School of Public Health, Harbin Medical University, Harbin 150081, China
  • Received:2020-05-07 Online:2021-01-10 Published:2021-01-12
  • Contact: HOU Shao-ying E-mail:hsy3982@163.com

摘要:

目的 研究糖尿病(DM)患者与糖尿病并发肺结核(DM-PTB)患者的血浆代谢组学之间的差异。方法 搜集2017年1—6月在黑龙江省传染病防治院确诊的DM患者(DM组)和DM-PTB患者(DM-PTB组),各10例。采用乙二胺四乙酸(EDTA)抗凝管收集研究对象清晨空腹静脉血10ml,并离心收集上清液。运用超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF MS)技术对两组患者的血浆样本进行代谢组学检测,之后采用多元统计分析方法比较其代谢图谱并筛选潜在的差异代谢产物。结果 DM组与DM-PTB组患者的血浆代谢图谱存在明显差异;初步鉴定7种在两组间有差异的代谢产物,其中,DM-PTB组血浆6-酮-前列腺素F1a(差异倍数143.34,P=0.020)、原卟啉原Ⅸ(差异倍数33.18,P=0.040)、亚油酸(差异倍数8.18,P=0.000)、5-羟基二十碳四烯酸(差异倍数15.09,P=0.000)、前列腺素E2(差异倍数8.88,P=0.000)和白三烯B4(差异倍数39.58,P=0.000)含量均高于DM组,而马尿酸含量(差异倍数2.69,P=0.010)低于DM组。结论 DM患者并发PTB后,体内花生四烯酸代谢、亚油酸代谢、氨基酸代谢发生改变,上述血浆差异代谢物可能是DM-PTB的早期筛查指标。

关键词: 糖尿病, 肺结核, 共病现象, 代谢组学, 血浆, 对比研究

Abstract:

Objective To explore the difference of plasma metabolomics between diabetes (DM) patients and diabetes complicated with tuberculosis (DM-PTB). Methods DM patients (DM group, n=10) and DM-PTB patients (DM-PTB group, n=10) diagnosed in Heilongjiang Provincial Infectious Disease Prevention and Treatment Hospital from January to June 2017 were collected. Venous blood were collected from these patients (10 ml per patient) with empty stomach in the early morning using Ethylenediaminetetraacetic acid (EDTA) anticoagulation tubes, and then the supernatant was collected by centrifugation. Metabolomics detection on the plasma samples of the two groups was performed by UPLC-Q-TOF MS technology, and then multivariate statistical analysis methods were used to compare their metabolic profiles and screen potential different metabolites. Results The plasma metabolism profiles of DM group and DM-PTB group were significantly different; 7 metabolites were preliminarily identified different between the two groups. Of them, the plasma 6-keto-prostaglandin F1a (multiple of difference 143.34, P=0.020), protoporphyrinogen Ⅸ (multiple of difference 33.18, P=0.040), linoleic acid (multiple of difference 8.18, P=0.000), 5-hydroxyeicosatetraenoic acid (multiple of difference 15.09, P=0.000), prostaglandin E2 (multiple of difference 8.88, P=0.000) and leukotriene B4 (multiple of difference 39.58, P=0.000) of the DM-PTB group were higher than those of the DM group, while hippuric acid content of the DM-PTB group (multiple of difference 2.69, P=0.010) was lower than that of the DM group. Conclusion Metabolism of arachidonic acid, linoleic acid, and amino acid happen changes after DM patients complicated with PTB, the above-mentioned plasma metabolites may be early screening indicators for DM-PTB.

Key words: Diabetes mellitus, Pulmonary tuberculosis, Comorbidity, Metabolomics, Plasma, Comparative study