中国防痨杂志 ›› 2020, Vol. 42 ›› Issue (8): 874-879.doi: 10.3969/j.issn.1000-6621.2020.08.018
收稿日期:
2020-04-18
出版日期:
2020-08-10
发布日期:
2020-08-10
通信作者:
周向梅
E-mail:zhouxm@cau.edu.cn
基金资助:
QU Meng-jin, LIANG Zheng-min, WANG Yuan-zhi, ZHOU Xiang-mei()
Received:
2020-04-18
Online:
2020-08-10
Published:
2020-08-10
Contact:
ZHOU Xiang-mei
E-mail:zhouxm@cau.edu.cn
摘要:
人们普遍认为结核分枝杆菌(Mycobacterium tuberculosis,MTB)优先依靠脂质代谢来建立和维持慢性感染,但是MTB的代谢网络可以对多种碳底物同时进行分解代谢,代谢的多功能性已经日益被认为是一种重要的致病机制。糖代谢在MTB的发病机制中占据重要地位,完整的糖代谢对于维持MTB稳态起着重要作用。作者以MTB糖代谢为线索,就MTB对糖类的转运、生成及分解代谢过程中的功能酶进行梳理和分析,并且探讨了糖代谢协助MTB维持稳态的机制,以期望为新型抗结核药品的研发提供科学依据。
屈孟锦, 梁正敏, 王元智, 周向梅. 结核分枝杆菌糖代谢的研究进展[J]. 中国防痨杂志, 2020, 42(8): 874-879. doi: 10.3969/j.issn.1000-6621.2020.08.018
QU Meng-jin, LIANG Zheng-min, WANG Yuan-zhi, ZHOU Xiang-mei. Research progress of carbohydrate metabolism of Mycobacterium tuberculosis[J]. Chinese Journal of Antituberculosis, 2020, 42(8): 874-879. doi: 10.3969/j.issn.1000-6621.2020.08.018
[1] |
Dheda K, Barry CE 3rd, Maartens G. Tuberculosis. Lancet, 2016,387(10024):1211-1226. doi: 10.1016/S0140-6736(15)00151-8.
doi: 10.1016/S0140-6736(15)00151-8 URL pmid: 26377143 |
[2] | World Health Organization. Global tuberculosis report 2019. Geneva: World Health Organization, 2019. |
[3] |
Lee W, VanderVen BC, Fahey RJ, et al. Intracellular Mycobacterium tuberculosis exploits host-derived fatty acids to limit metabolic stress. J Biol Chem, 2013,288(10):6788-6800. doi: 10.1074/jbc.M112.445056.
URL pmid: 23306194 |
[4] |
Pandey AK, Sassetti CM. Mycobacterial persistence requires the utilization of host cholesterol. Proc Natl Acad Sci U S A, 2008,105(11):4376-4380. doi: 10.1073/pnas.0711159105.
doi: 10.1073/pnas.0711159105 URL pmid: 18334639 |
[5] |
Ehrt S, Schnappinger D, Rhee KY. Metabolic principles of persistence and pathogenicity in Mycobacterium tuberculosis. Nat Rev Microbiol, 2018,16(8):496-507. doi: 10.1038/s41579-018-0013-4.
doi: 10.1038/s41579-018-0013-4 URL pmid: 29691481 |
[6] |
Cumming BM, Steyn AJ. Metabolic plasticity of central carbon metabolism protects mycobacteria. Proc Natl Acad Sci U S A, 2015,112(43):13135-13136. doi: 10.1073/pnas.1518171112.
doi: 10.1073/pnas.1518171112 URL pmid: 26483480 |
[7] |
Sia JK, Rengarajan J. Immunology of Mycobacterium tuberculosis Infections. Microbiol Spectr, 2019, 7(4):10.1128/microbiolspec.GPP3-0022-2018. doi: 10.1128/microbiolspec.GPP3-0022-2018.
doi: 10.1128/microbiolspec.GPP3-0053-2018 URL pmid: 31298205 |
[8] |
Shi L, Sohaskey CD, Pheiffer C, et al. Carbon flux rerouting during Mycobacterium tuberculosis growth arrest. Mol Microbiol, 2010,78(5):1199-1215. doi: 10.1111/j.1365-2958.2010.07399.x.
doi: 10.1111/j.1365-2958.2010.07399.x URL pmid: 21091505 |
[9] |
Cole ST, Brosch R, Parkhill J, et al. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature, 1998,393(6685):537-544. doi: 10.1038/31159.
doi: 10.1038/31159 URL pmid: 9634230 |
[10] |
Titgemeyer F, Amon J, Parche S, et al. A genomic view of sugar transport in Mycobacterium smegmatis and Mycobacterium tuberculosis. J Bacteriol, 2007,189(16):5903-5915. doi: 10.1128/JB.00257-07.
doi: 10.1128/JB.00257-07 URL pmid: 17557815 |
[11] |
Sassetti CM, Rubin EJ. Genetic requirements for mycobacterial survival during infection. Proc Natl Acad Sci U S A, 2003,100(22):12989-12994. doi: 10.1073/pnas.2134250100.
doi: 10.1073/pnas.2134250100 URL pmid: 14569030 |
[12] |
Soni DK, Dubey SK, Bhatnagar R. ATP-binding cassette (ABC) import systems of Mycobacterium tuberculosis: target for drug and vaccine development. Emerg Microbes Infect, 2020,9(1):207-220. doi: 10.1080/22221751.2020.1714488.
doi: 10.1080/22221751.2020.1714488 URL pmid: 31985348 |
[13] |
Jiang D, Zhang Q, Zheng Q, et al. Structural analysis of Mycobacterium tuberculosis ATP-binding cassette transporter subunit UgpB reveals specificity for glycerophosphocholine. FEBS J, 2014,281(1):331-341. doi: 10.1111/febs.12600.
doi: 10.1111/febs.12600 URL pmid: 24299297 |
[14] |
Kalscheuer R, Weinrick B, Veeraraghavan U, et al. Trehalose-recycling ABC transporter LpqY-SugA-SugB-SugC is essential for virulence of Mycobacterium tuberculosis. Proc Natl Acad Sci U S A, 2010,107(50):21761-21766. doi: 10.1073/pnas.1014642108.
doi: 10.1073/pnas.1014642108 URL pmid: 21118978 |
[15] |
Fullam E, Prokes I, Futterer K, et al. Structural and functional analysis of the solute-binding protein UspC from Mycobacterium tuberculosis that is specific for amino sugars. Open Biology, 2016,6(6):160105. doi: 10.1098/rsob.160105.
doi: 10.1098/rsob.160105 URL pmid: 27335320 |
[16] |
Shin SJ, Kim SY, Shin AR, et al. Identification of Rv2041c, a novel immunogenic antigen from Mycobacterium tuberculosis with serodiagnostic potential. Scandinavian journal of immunology, 2009,70(5):457-464. doi: 10.1111/j.1365-3083.2009.02324.x.
doi: 10.1111/j.1365-3083.2009.02324.x URL pmid: 19874550 |
[17] |
Muñoz-Elías EJ McKinney JD. Mycobacterium tuberculosis isocitrate lyases 1 and 2 are jointly required for in vivo growth and virulence. Nat Med, 2005,11(6):638-644. doi: 10.1038/nm1252.
doi: 10.1038/nm1252 URL pmid: 15895072 |
[18] |
Basu P, Sandhu N, Bhatt A, et al. The anaplerotic node is essential for the intracellular survival of Mycobacterium tuberculosis. J Biol Chem, 2018,293(15):5695-5704. doi: 10.1074/jbc.RA118.001839.
doi: 10.1074/jbc.RA118.001839 URL pmid: 29475946 |
[19] |
Marrero J, Rhee KY, Schnappinger D, et al. Gluconeogenic carbon flow of tricarboxylic acid cycle intermediates is critical for Mycobacterium tuberculosis to establish and maintain infection. Proc Natl Acad Sci U S A, 2010,107(21):9819-9824. doi: 10.1073/pnas.1000715107.
doi: 10.1073/pnas.1000715107 URL pmid: 20439709 |
[20] |
Puckett S, Trujillo C, Eoh H, et al. Inactivation of fructose-1,6-bisphosphate aldolase prevents optimal co-catabolism of glycolytic and gluconeogenic carbon substrates in Mycobacterium tuberculosis. PLoS Pathog, 2014,10(5):e1004144. doi: 10.1371/journal.ppat.1004144.
doi: 10.1371/journal.ppat.1004144 URL pmid: 24851864 |
[21] |
Trujillo C, Blumenthal A, Marrero J, et al. Triosephosphate isomerase is dispensable in vitro yet essential for Mycobacterium tuberculosis to establish infection. mBio, 2014,5(2):e00085. doi: 10.1128/mBio.00085-14.
doi: 10.1128/mBio.00085-14 URL pmid: 24757211 |
[22] |
Ganapathy U, Marrero J, Calhoun S, et al. Two enzymes with redundant fructose bisphosphatase activity sustain gluconeogenesis and virulence in Mycobacterium tuberculosis. Nature communications, 2015,6:7912. doi: 10.1038/ncomms8912.
doi: 10.1038/ncomms8912 URL pmid: 26258286 |
[23] |
Marrero J, Trujillo C, Rhee KY, et al. Glucose phosphorylation is required for Mycobacterium tuberculosis persistence in mice. PLoS Pathog, 2013,9(1):e1003116. doi: 10.1371/journal.ppat.1003116.
doi: 10.1371/journal.ppat.1003116 URL pmid: 23326232 |
[24] |
Phong WY, Lin W, Rao SP, et al. Characterization of phosphofructokinase activity in Mycobacterium tuberculosis reveals that a functional glycolytic carbon flow is necessary to limit the accumulation of toxic metabolic intermediates under hypoxia. PLoS One, 2013,8(2):e56037. doi: 10.1371/journal.pone.0056037.
doi: 10.1371/journal.pone.0056037 URL pmid: 23409118 |
[25] |
Arroyo L, Rojas M, Franken KL, et al. Multifunctional T Cell Response to DosR and Rpf Antigens Is Associated with Protection in Long-Term Mycobacterium tuberculosis-Infected Individuals in Colombia. Clin Vaccine Immunol, 2016,23(10):813-824. doi: 10.1128/CVI.00217-16.
doi: 10.1128/CVI.00217-16 URL pmid: 27489136 |
[26] |
de la Paz Santangelo M, Gest PM, Guerin ME, et al. Glycolytic and non-glycolytic functions of Mycobacterium tuberculosis fructose-1,6-bisphosphate aldolase, an essential enzyme produced by replicating and non-replicating bacilli. J Biol Chem, 2011,286(46):40219-40231. doi: 10.1074/jbc.M111.259440.
doi: 10.1074/jbc.M111.259440 URL pmid: 21949126 |
[27] |
Grüning NM, Du D, Keller MA, et al. Inhibition of triosephosphate isomerase by phosphoenolpyruvate in the feedback-regulation of glycolysis. Open Biol, 2014,4(3):130232. doi: 10.1098/rsob.130232.
doi: 10.1098/rsob.130232 URL |
[28] |
Noy T, Vergnolle O, Hartman TE, et al. Central Role of Pyruvate Kinase in Carbon Co-catabolism of Mycobacterium tuberculosis. J Biol Chem, 2016,291(13):7060-7069. doi: 10.1074/jbc.M115.707430.
doi: 10.1074/jbc.M115.707430 URL pmid: 26858255 |
[29] |
Chavadi S, Wooff E, Coldham NG, et al. Global effects of inactivation of the pyruvate kinase gene in the Mycobacterium tuberculosis complex. J Bacteriol, 2009,191(24):7545-7553. doi: 10.1128/JB.00619-09.
doi: 10.1128/JB.00619-09 URL pmid: 19820096 |
[30] |
Zhong W, Guo J, Cui L, et al. Pyruvate Kinase Regulates the Pentose-Phosphate Pathway in Response to Hypoxia in Mycobacterium tuberculosis. J Mol Biol, 2019,431(19):3690-3705. doi: 10.1016/j.jmb.2019.07.033.
doi: 10.1016/j.jmb.2019.07.033 URL pmid: 31381898 |
[31] |
Snásˇel J, Pichová I. Allosteric regulation of pyruvate kinase from Mycobacterium tuberculosis by metabolites. Biochim Biophys Acta Proteins Proteom, 2019,1867(2):125-139. doi: 10.1016/j.bbapap.2018.11.002.
doi: 10.1016/j.bbapap.2018.11.002 URL pmid: 30419357 |
[32] |
Howard NC, Khader SA. Immunometabolism during Mycobacterium tuberculosis Infection. Trends Microbiol, 2020: S0966-842X(20)30103-7. doi: 10.1016/j.tim.2020.04.010.
doi: 10.1016/j.tim.2020.02.006 URL pmid: 32544442 |
[33] |
Shi L, Eugenin EA, Subbian S. Immunometabolism in Tuberculosis. Front Immunol, 2016,7:150. doi: 10.3389/fimmu.2016.00150.
doi: 10.3389/fimmu.2016.00150 URL pmid: 27148269 |
[34] |
Shi L, Jiang Q, Bushkin Y, et al. Biphasic Dynamics of Macrophage Immunometabolism during Mycobacterium tuberculosis Infection. mBio, 2019,10(2):e02550-18. doi: 10.1128/mBio.02550-18.
doi: 10.1128/mBio.02550-18 URL pmid: 30914513 |
[35] |
Rizvi A, Shankar A, Chatterjee A, et al. Rewiring of Metabolic Network in Mycobacterium tuberculosis During Adaptation to Different Stresses. Front Microbiol, 2019,10:2417. doi: 10.3389/fmicb.2019.02417.
doi: 10.3389/fmicb.2019.02417 URL pmid: 31736886 |
[36] |
Russell DG, Huang L, VanderVen BC. Immunometabolism at the interface between macrophages and pathogens. Nat Rev Immunol, 2019,19(5):291-304. doi: 10.1038/s41577-019-0124-9.
doi: 10.1038/s41577-019-0124-9 URL pmid: 30679807 |
[37] |
Koul A, Vranckx L, Dhar N, et al. Delayed bactericidal response of Mycobacterium tuberculosis to bedaquiline involves remodelling of bacterial metabolism. Nat Commun, 2014,5:3369. doi: 10.1038/ncomms4369.
doi: 10.1038/ncomms4369 URL pmid: 24569628 |
[38] |
Stokes JM, Lopatkin AJ, Lobritz MA, et al. Bacterial Metabo-lism and Antibiotic Efficacy. Cell Metab, 2019,30(2):251-259. doi: 10.1016/j.cmet.2019.06.009.
doi: 10.1016/j.cmet.2019.06.009 URL pmid: 31279676 |
[39] |
Zhang F, Li S, Wen S, et al. Comparison of in vitro Susceptibility of Mycobacteria Against PA-824 to Identify Key Residues of Ddn, the Deazoflavin-Dependent Nitroreductase from Mycobacterium tuberculosis. Infect Drug Resist, 2020,13:815-822. doi: 10.2147/IDR.S240716.
doi: 10.2147/IDR.S240716 URL pmid: 32210596 |
[40] |
Pethe K, Bifani P, Jang J, et al. Discovery of Q203, a potent clinical candidate for the treatment of tuberculosis. Nat Med, 2013,19(9):1157-1160. doi: 10.1038/nm.3262.
doi: 10.1038/nm.3262 URL pmid: 23913123 |
[41] |
Korte J, Alber M, Trujillo CM, et al. Trehalose-6-Phosphate-Mediated Toxicity Determines Essentiality of OtsB2 in Mycobacterium tuberculosis In Vitro and in Mice. PLoS Pathog, 2016,12(12):e1006043. doi: 10.1371/journal.ppat.1006043.
doi: 10.1371/journal.ppat.1006043 URL pmid: 27936238 |
[42] |
Eoh H, Wang Z, Layre E, et al. Metabolic anticipation in Mycobacterium tuberculosis. Nat Microbiol, 2017,2:17084. doi: 10.1038/nmicrobiol.2017.84.
URL pmid: 28530656 |
[43] |
Zhong W, Cui L, Goh BC, et al. Allosteric pyruvate kinase-based “logic gate” synergistically senses energy and sugar levels in Mycobacterium tuberculosis. Nat Commun, 2017,8(1):1986. doi: 10.1038/s41467-017-02086-y.
doi: 10.1038/s41467-017-02086-y URL pmid: 29215013 |
[44] |
Stincone A, Prigione A, Cramer T, et al. The return of metabolism: biochemistry and physiology of the pentose phosphate pathway. Biol Rev Camb Philos Soc, 2015,90(3):927-963. doi: 10.1111/brv.12140.
doi: 10.1111/brv.12140 URL pmid: 25243985 |
[45] |
Chao WC, Yen CL, Hsieh CY, et al. Mycobacterial infection induces higher interleukin-1β and dysregulated lung inflammation in mice with defective leukocyte NADPH oxidase. PLoS One, 2017,12(12):e0189453. doi: 10.1371/journal.pone.0189453.
doi: 10.1371/journal.pone.0189453 URL pmid: 29228045 |
[46] |
Mehrotra P, Jamwal SV, Saquib N, et al. Pathogenicity of Mycobacterium tuberculosis is expressed by regulating metabolic thresholds of the host macrophage. PLoS Pathog, 2014,10(7):e1004265. doi: 10.1371/journal.ppat.1004265.
doi: 10.1371/journal.ppat.1004265 URL pmid: 25058590 |
[1] | 周林, 刘二勇, 孟庆琳, 陈明亭, 周新华, 高微微, 林明贵, 谢汝明. 《WS 288—2017 肺结核诊断》标准实施后肺结核诊断质量评估分析[J]. 中国防痨杂志, 2020, 42(9): 910-915. |
[2] | 王前, 周林, 刘二勇, 赵雁林, 李涛, 陈明亭, 杨丽佳, 王嘉. 我国县级结核病定点医疗机构结核病诊断能力现况调查研究[J]. 中国防痨杂志, 2020, 42(9): 926-930. |
[3] | 苏茜, 夏勇, 逯嘉, 王丹霞, 何金戈. 2009—2018年四川省0~14 岁儿童肺结核流行特征分析[J]. 中国防痨杂志, 2020, 42(9): 942-947. |
[4] | 邓亚丽, 张天华, 刘卫平, 张宏伟, 马煜, 李鹏. 2014—2018年陕西省肺结核发病的时空聚集性分析[J]. 中国防痨杂志, 2020, 42(9): 948-955. |
[5] | 梁瑞云, 方伟军, 任会丽, 黎惠如, 张晖. 非结核分枝杆菌肺病并发与未并发糖尿病患者的CT征象研究[J]. 中国防痨杂志, 2020, 42(9): 962-967. |
[6] | 马廷龙, 韩毅, 程序, 刘志东. 超声抗结核药品电导入联合化疗对胸壁结核的疗效观察[J]. 中国防痨杂志, 2020, 42(9): 968-972. |
[7] | 张丽娟, 陶晓, 夏丽莉, 魏芬芬, 郑琦. 脊柱结核患者围手术期应用加速康复集束化护理临床路径表的价值[J]. 中国防痨杂志, 2020, 42(9): 981-986. |
[8] | 孙海燕, 李烁, 王忠东, 任志盛, 宋颂, 薛白, 张华强, 代晓琦. 2010—2019年青岛市学生肺结核流行病学特征分析[J]. 中国防痨杂志, 2020, 42(9): 994-997. |
[9] | 李爱芳, 崔晓利, 康磊, 雷静, 党丽云, 杨翰. 荧光PCR探针熔解曲线法检测结核分枝杆菌耐药性的价值[J]. 中国防痨杂志, 2020, 42(9): 998-1001. |
[10] | 中国防痨协会 中国防痨协会学校与儿童结核病防治专业分会 《中国防痨杂志》编辑委员会. 重组结核杆菌融合蛋白(EC)临床应用专家共识[J]. 中国防痨杂志, 2020, 42(8): 761-768. |
[11] | 杨蕾, 韦芬, 张凯, 仇晶晶, 汪莹莹, 都伟欣, 卢锦标, 陶立峰, 蒲江. 重组结核杆菌融合蛋白(EC)的稳定性与有效性研究[J]. 中国防痨杂志, 2020, 42(8): 799-806. |
[12] | 张凯, 沈小兵, 陶立峰, 韦芬, 陈保文, 仇晶晶, 陈伟, 卢锦标, 朱银猛, 程兴, 钟再新, 赵爱华, 蒲江. 重组结核杆菌融合蛋白(EC)产品质量标准的建立[J]. 中国防痨杂志, 2020, 42(8): 814-820. |
[13] | 陈凤芳, 马俊, 黄劲, 尹洪云, 沙巍, 杨光红, 冯永红. 建立结核感染T淋巴细胞斑点试验阳性的纵隔淋巴结结核与Ⅰ/Ⅱ期结节病诊断模型初探[J]. 中国防痨杂志, 2020, 42(8): 832-837. |
[14] | 盛杰, 朱洋, 地里下提·阿不力孜, 唐伟, 古甫丁, 宋兴华. 表型与分子药物敏感性试验对术后耐药骨关节结核患者化疗的指导与效果分析[J]. 中国防痨杂志, 2020, 42(8): 838-844. |
[15] | 赵铁牛, 姜爽爽, 黄莉, 胡雪梅. 多层螺旋CT与彩色多普勒超声检查辅助诊断骨与关节结核的对比研究[J]. 中国防痨杂志, 2020, 42(8): 845-849. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||