关键词: 结核, 肺, 糖尿病, 2型, 共病现象, 氧化性应激, 血液学试验, 对比研究

Abstract:

Objective To explore the profile of oxidative stress in plasma of patients with pulmonary tuberculosis complicated with type 2 diabetes mellitus.Methods Forty five cases of tuberculosis patients (group PTB), 28 cases of tuberculosis and type 2 diabetes (group PTB-DM2), and 24 healthy volunteers (group HC) were collected from February 2016 to January 2017 in the Shanghai Public Health Clinical Center. The content of total antioxidant capacity (T-AOC), catalase (CAT), heme oxygenase-1 (HO-1), glutathione (GSH), superoxide dismutase (SOD) and lipid malondialdehyde (MDA) in the 3 groups were measured, respectively, with the median (quartile) (M (P₂₅, P₇₅)). SPSS 23.0 software was used to analyze the data of 3 groups, and GraphPad Prism 7.0 software was used to map, the difference was statistically significant in P<0.05.Results The biochemical indexes of group HC, group PTB, and group PTB-DM2 were T-AOC (4.65 (4.16, 5.15), 2.70 (3.50, 4.30), 2.55 (2.03,3.48) U/ml), CAT (54.20 (35.46,81.30), 23.49 (9.49, 44.72), 5.66 (-14.00, 31.98) U/ml), HO-1 (16.12 (11.84, 24.09), 11.44 (7.95, 15.53), 8.19 (7.53, 11.58) ng/ml), GSH (5.14 (3.98, 7.33), 4.69 (3.02, 6.47), 2.90 (1.90, 6.14) μmol/ml), SOD (14.31 (10.63, 17.33), 14.28 (11.86, 15.69), 13.78 (12.26, 18.00) U/ml), MDA (3.60 (2.62, 4.40), 5.11 (4.26, 7.23), 12.77 (9.47, 14.89) nmol/ml), there were significant differences between the three groups (χ ²=35.28,P<0.01;χ ²=28.94,P<0.01;χ ²=23.00,P<0.01;χ ²=9.24,P=0.010;χ ²=15.53,P<0.01;χ ²=59.46,P<0.01). The plasma levels of T-AOC, CAT and HO-1 in the PTB group were significantly lower than those in the HC group, and the MDA level was significantly higher than that in the HC group (Z=-3.88,P<0.01; Z=-3.82,P<0.01;Z=-3.21,P=0.005;Z=-3.94,P=0.008, respectively). The levels of T-AOC, CAT, HO-1, GSH and SOD in the PTB-DM2 group were all significantly lower than those of the PTB patients, and the MDA level was significantly higher than those of the PTB patients (Z=-3.08, P=0.047; Z=-2.44, P=0.046; Z=-2.27, P=0.023; Z=-2.45, P=0.096; Z=-3.50, P=0.002; Z=-6.01, P<0.01). Conclusion After infection of MTB, the anti-oxidant ability of PTB patients is lower and the lipid oxidation damage is present. While combined type 2 diabetes, the oxidative stress of PTB patients can be aggravated, the patient’s antioxidant capacity is lower, and the lipid oxidation damage is aggravated.

Key words: Tuberculosis, pulmonary, Diabetes mellitus, type 2, Comorbidity, Oxidative stress, Hematologic tests, Comparative study