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中国防痨杂志 ›› 2010, Vol. 32 ›› Issue (12): 801-805.

• 论著 • 上一篇    下一篇

γ-干扰素释放试验(IGRA)的诊断价值探讨(高感染率背景下的研究结果)

刘旭晖; 乐军; 张忠顺; 肖和平;   

  1. 同济医科大学附属上海市肺科医院;
  • 出版日期:2010-12-15 发布日期:2011-08-23
  • 基金资助:

    全球基金中国结核病实施性研究《结核病院内感染研究》(TB08-006)

Diagnostic value of interferon gamma release assay(IGRA) in high incidence background

Liu Xuhui; Yue Jun; Zhang Zhongshun; Xiao Heping   

  1. Department of tuberculosis; Shanghai Pulmonary Hospital; Shanghai 200433; China;
  • Online:2010-12-15 Published:2011-08-23

摘要: 目的评估在中国结核分枝杆菌高感染率背景下,γ-干扰素释放试验(interferon gammarelease assay,IGRA)对于诊断结核病的价值。方法以上海市肺科医院2005—2010年完成的201例γ-干扰素释放试验(T-SPOT.TB)及后续随访结果为研究资料,其中结核组(A组)69例、结核科医务人员组(B组)56例、肺癌组(C组)22例、普通肺炎组(D组)20例、健康对照组(E组)30例。应用T-SPOT.TB(Oxford Immunotec,Abingdon,UK)试剂盒检测外周静脉血淋巴细胞对于2种结核分枝杆菌菌种特异性分泌蛋白:早期分泌靶抗原6(ESAT-6)和培养分泌蛋白10(CFP-10)的免疫反应。结果 A组阳性率91.3%(63/69),B组阳性率17.9%(10/56),C组阳性率27.3%(6/22),D组阳性率10%(2/20),E组阳性率6.7%(2/30)。A和C组阳性率较E组高,差异有统计学意义(P<0.05);B和D组较E组差异无统计学意义,但有升高的趋势。B组IGRA阳性率较E组显著升高,取中位工作年限3年为分界线,工作年限大于3年者IGRA阳性率27.6%(8/...

关键词: 结核/诊断, 干扰素Ⅱ型, 免疫酶技术

Abstract: Objective To evaluate the diagnostic value of interferon gamma release assay(IGRA) in high incidence background.Methods Two hundred and one cases from Shanghai pulmonary hospital were involved in this study,including 69 patients with active tuberculosis(group A),60 health-care workers from department of tuberculosis(group B),22 lung cancer patients(group C),20 pneumonia patients(group D),30 health controls(group E).Blood samples were detected the reactions of stimulated T-cells releasing interferon gamma by...

Key words: tuberculosis/diagnosis, interferm type Ⅱ, immunoenzyme techniques

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