加强结核病耐药性检测研究提高正确解读检测结果水平

doi:10.3969/j.issn.1000-6621.2019.02.002

摘要/Abstract

吴雪琼. 加强结核病耐药性检测研究提高正确解读检测结果水平[J]. 中国防痨杂志, 2019, 41(2): 124-128. doi: 10.3969/j.issn.1000-6621.2019.02.002

图/表 1

参考文献 35

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类别技术与方法	检测药物种类	检测特点	所需检测时间	自动化程度	操作步骤	生物安全要求
表型DST
绝对浓度法	4种一线和12种二线药物	可了解高、低水平耐药情况	平均30d	手工操作	样本处理、接种、培养	BSL-Ⅱ
比例法	4种一线和12种二线药物	含药管菌落数>1%判为耐药	平均30d	手工操作	样本处理、接种、培养	BSL-Ⅱ
快培系统	RFP、INH、Sm、EMB、和PZA	间接检测MTB生长代谢情况	平均8~10d	手工操作、自动判读结果	样本处理、接种、培养	BSL-Ⅱ
微孔板法	7或5种一线和8或7种二线药物	了解药物MICs	7~10d	手工操作、自动判读结果	样本处理、接种、培养	BSL-Ⅱ
分子DST
实时荧光定量PCR	RFP	不报告具体的突变类型	2h	核酸提取、扩增、检测自动化	简便、快速、自动化	样本处理 BSL-Ⅱ
探针熔解曲线法	RFP、INH、Sm、EMB、和FQs	不报告具体的突变类型	2~3h	手工操作、通用荧光定量PCR仪	简便、快速、闭管检测	核酸提取 BSL-Ⅱ
基因芯片技术	RFP、INH	了解突变的位点和性质	1~2d	配套仪器、自动判读结果	杂交、检测过程较繁琐	核酸提取 BSL-Ⅱ
反向杂交技术	RFP、INH、FQs、Km、Am和Cm	了解突变的位点和性质	1~2d	配套仪器、自动判读结果	杂交、检测过程较繁琐	核酸提取 BSL-Ⅱ

加强结核病耐药性检测研究提高正确解读检测结果水平

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