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中国防痨杂志 ›› 2018, Vol. 40 ›› Issue (8): 866-871.doi: 10.3969/j.issn.1000-6621.2018.08.016

• 论著 • 上一篇    下一篇

兔布鲁杆菌病性脊柱炎动物模型的建立

荀传辉,蔡晓宇,王传锋,买尔旦·买买提()   

  1. 新疆维吾尔自治区伊犁职业技术学院(王传锋)
  • 收稿日期:2018-05-15 出版日期:2018-08-10 发布日期:2018-09-09
  • 通信作者: 荀传辉,买尔旦·买买提 E-mail:mardanmmtmx@163.com
  • 基金资助:
    新疆维吾尔自治区重点研发计划(2016B03047-3)

Establishment of a rabbit model of brucellar spondylitis

Chuan-hui XUN,Xiao-yu CAI,Chuan-feng WANG, ()   

  1. *Department of Spinal Surgery,the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China
  • Received:2018-05-15 Online:2018-08-10 Published:2018-09-09
  • Contact: Chuan-hui XUN, E-mail:mardanmmtmx@163.com

摘要:

目的 探讨使用构建兔脊柱结核模型的相同方法用以构建新西兰兔布鲁杆菌病性脊柱炎动物模型,并对其可行性进行评价。方法 48只新西兰兔采用数表法随机分为实验组16只、对照组16只、空白组16只。实验组于第6腰椎(L6)上终板下方钻孔,填入明胶海绵,在其中浸注种植M5羊种布鲁杆菌弱毒苗混悬液0.1ml(3×10 8CFU/ml);对照组浸注0.9% NaCl溶液0.1ml;空白组不做任何处理。 结果 术后8周兔存活率实验组为75.0% (12/16)、对照组为87.5% (14/16),空白组为100.0% (16/16)。实验组术后4周CT扫描三维重建结果显示5只出现椎间隙变窄,MRI检查结果显示7只有椎旁软组织脓肿形成;对照组未出现椎旁软组织脓肿形成,椎间隙无变化。实验组术后8周行CT扫描三维重建复查,结果显示8只出现椎间隙变窄,MRI检查结果显示10只出现椎旁软组织脓肿形成及椎间盘信号改变;对照组未见椎旁软组织脓肿形成及椎间盘信号改变。实验组术后8周进行组织病理学检查,显示兔椎旁软组织均有病变组织细胞增生及以淋巴细胞为主的炎性细胞浸润;对照组与空白组未出现病变组织细胞增生及炎性细胞浸润。结论 在新西兰兔腰椎椎体的上终板下钻孔、明胶海绵填塞、浸注种植M5羊种布鲁杆菌弱毒苗、石蜡封闭孔道的方法,可成功构建兔布鲁杆菌病性脊柱炎动物模型。

关键词: 布鲁杆菌病, 脊柱炎, 疾病模型, 动物, 兔, 方法

Abstract:

Objective To explore the feasibility of establishing a New Zealand rabbit Brucellosis spondylitis animal model based on the method used in constructing a rabbit spinal tuberculosis model.Methods Forty-eight New Zealand rabbits were randomly divided into an experimental group (16), a control group (16) and a blank group (16). In the experimental group, a gelatin sponge containing 0.1 ml of a M5 suspension of an attenuated sheep Brucella vaccine (3×10 8 CFU/ml) was inserted in a hole drilled under the endplate of L6. In the control group, the gelatin sponge was immersed in 0.1 ml of a 0.9% NaCl solution. The blank group did not receive any treatment. Results Eight weeks after surgery, the survival rate of the rabbits in the experimental group was 75.0% (12/16), that in the control group was 87.5% (14/16), and that in the blank group was 100.0% (16/16). Four weeks after surgery, X-ray and CT 3D reconstruction results showed the intervertebral space in 5 rabbits from the experimental group became narrowed, and MRI examination showed that 7 rabbits had paravertebral soft tissue abscess formation. There was no paravertebral soft tissue abscess formation in the control group and the intervertebral disc showed no change. Eight weeks after surgery, X-ray and CT 3D reconstruction showed narrowing of the intervertebral space in 8 rabbits, and MRI examination showed paravertebral soft tissue abscess formation and disc signal change in 10 rabbits. Paravertebral soft tissue abscess formation and disc signal change was not observed in the control group. Histopathology analysis at 8 weeks after surgery showed pathological changes in the paravertebral soft tissue in the experimental group and the infiltration of inflammatory cells (predominantly lymphocytes). No changes in cell proliferation and inflammatory cell infiltration were found in the control group nor the blank group.Conclusion A new animal model of Brucellosis spondylitis can be established by drilling a hole in the upper endplate of the lumbar vertebrae, applying a gelatin sponge injected with an M5 suspension of the attenuated sheep Brucella vaccine and sealing the whole with paraffin wax.

Key words: Brucellosis, Spondylitis, Disease models, animal, Rabbits, Methods