重视结核分枝杆菌抗原检测技术研发

doi:10.19982/j.issn.1000-6621.20210558

摘要/Abstract

摘要：

结核病疫情防控面临的新形势需要考虑新的快速、便捷的早期筛查或诊断技术。简便、快速和廉价的免疫学诊断技术是结核病研究人员重点关注并且坚持不懈的努力方向。目前结核分枝杆菌抗原靶标包括单一抗原的检测和多种抗原同时检测,涉及的检测技术包括免疫学技术、质谱技术及纳米技术。随着技术的进步,结核分枝杆菌抗原在检测技术和研究思路上都取得了较大的进展,但仍存在一些问题。结核分枝杆菌抗原研发的方向应注重多种特异性抗原同时检测。随着新型纳米材料和传感方法的发展,特异性强、免疫原性好的抗原得到不断发现和深入研究,这将有助于结核分枝杆菌抗原检测得到新的完善和发展。

关键词: 分枝杆菌,结核, 抗原, 诊断技术和方法, 总结性报告(主题)

Abstract:

New fast and convenient early screening or diagnosis technologies are required because of new situation of tuberculosis epidemic prevention and control. Simple, fast and cheap immunological diagnosis technology is the focus and persistent efforts of tuberculosis researchers. At present, the antigen targets of Mycobacterium tuberculosis (MTB) include the detection of single antigen and the simultaneous detection of multiple antigens. The involved detection technologies include immunological technology, mass spectrometry technology and nanotechnology. With the development of technology, great progress has been made in detection technology and research ideas of MTB antigen. However, there are still some problems. The simultaneous detection of multiple specific antigens should be deeply developed in research of MTB antigen. The development of new nanomaterials and sensing methods, as well as the discovery and research of antigens with strong specificity and good immunogenicity, are help to get new improvement and development of MTB antigen detection method.

Key words: Mycobacterium tuberculosis, Antigens, Diagnostic techniques and procedures, Consensus development conferences as topic

中图分类号:

R378.91
R392

孙照刚. 重视结核分枝杆菌抗原检测技术研发[J]. 中国防痨杂志, 2022, 44(2): 120-124. doi: 10.19982/j.issn.1000-6621.20210558

SUN Zhao-gang. Attention should be paid to the research and development of Mycobacterium tuberculosis antigen detection technology[J]. Chinese Journal of Antituberculosis, 2022, 44(2): 120-124. doi: 10.19982/j.issn.1000-6621.20210558

参考文献 29

[1]	World Health Organization. ZH mediacentre PR 2011: WHO warns against the use of inaccurate blood tests for active tuberculosis. Geneva: World Health Organization, 2011.
[2]	Silva VM, Kanaujia G, Gennaro ML, et al. Factors associated with humoral response to ESAT-6, 38 kDa and 14 kDa in patients with a spectrum of tuberculosis. Int J Tuberc Lung Dis, 2003, 7(5):478-484. pmid: 12757050
[3]	Bera S, Shende N, Kumar S, et al. Detection of antigen and antibody in childhood tuberculous meningitis. Indian J Pediatr, 2006, 73(8):675-679. doi: 10.1007/BF02898442. doi: 10.1007/BF02898442 URL
[4]	樊学军, 罗垲炜, 田绿波, 等. 重组38kDa抗原对结核病诊断应用价值的评价. 中国医药指南, 2010, 8(34):177-178.
[5]	唐宇龙, 杨华, 刘湘新, 等. 三种结核分枝杆菌分泌蛋白的抗体制备及其在抗原检测中的应用. 中华传染病杂志, 2007, 25(10):597-600. doi: 10.3760/j.issn:1000-6680.2007.10.005. doi: 10.3760/j.issn:1000-6680.2007.10.005
[6]	Mudaliar AV, Kashyap RS, Purohit HJ, et al. Detection of 65 kD heat shock protein in cerebrospinal fluid of tuberculous meningitis patients. BMC Neurol, 2006, 6:34. doi: 10.1186/1471-2377-6-34. doi: 10.1186/1471-2377-6-34 pmid: 16978411
[7]	梁正敏, 王元智, 刘一朵, 等. 抗原85复合物的致病机制及其在结核病疫苗研制中的应用进展. 中国防痨杂志, 2020, 42(11):1243-1249. doi: 10.3969/j.issn.1000-6621.2020.11.018. doi: 10.3969/j.issn.1000-6621.2020.11.018
[8]	Khan A, Singh R, Sharma S, et al. Diagnosis of osteoarticular tuberculosis by immuno-PCR assay based on mycobacterial antigen 85 complex detection. Lett Appl Microbiol, 2021, 74(1):17-26. doi: 10.1111/lam.13567. doi: 10.1111/lam.13567 URL
[9]	李荣娟. 血清脂阿拉伯甘露糖-IgG诊断肺结核的临床价值. 山东医学高等专科学校学报, 2021, 43(4):293-294. doi: 10.3969/j.issn.1674-0947.2021.04.023. doi: 10.3969/j.issn.1674-0947.2021.04.023
[10]	Flores LL, Steingart KR, Dendukuri N, et al. Systematic review and meta-analysis of antigen detection tests for the diagnosis of tuberculosis. Clin Vaccine Immunol, 2011, 18(10):1616-1627. doi: 10.1128/CVI.05205-11. doi: 10.1128/CVI.05205-11 pmid: 21832100
[11]	Iskandar A, Nursiloningrum E, Arthamin MZ, et al. The diagnostic value of urine lipoarabinomannan (LAM) antigen in childhood tuberculosis. J Clin Diagn Res, 2017, 11(3): EC32-EC35. doi: 10.7860/JCDR/2017/20909.9542. doi: 10.7860/JCDR/2017/20909.9542
[12]	Wu X, Yang Y, Zhang J, et al. Comparison of antibody responses to seventeen antigens from Mycobacterium tuberculosis. Clin Chim Acta, 2010, 411(19/20):1520-1528. doi: 10.1016/j.cca.2010.06.014. doi: 10.1016/j.cca.2010.06.014 URL
[13]	杨群, 江晓静. 结核蛋白芯片对肺结核及肺外结核的诊断价值. 西安交通大学学报(医学版), 2012, 33(1): 129-130, 封3. doi: 10.3969/j.issn.1671-8259.2012.01.032. doi: 10.3969/j.issn.1671-8259.2012.01.032
[14]	Zhou F, Xu X, Wu S, et al. Protein array identification of protein markers for serodiagnosis of Mycobacterium tuberculosis infection. Sci Rep, 2015, 5:15349. doi: 10.1038/srep15349. doi: 10.1038/srep15349 URL
[15]	贺仁忠, 王霄, 陈玲, 等. 结核潜伏感染诊断新型候选标志物的筛选及临床验证. 中国人兽共患病学报, 2018, 34(9):794-800. doi: 10.3969/j.issn.1002-2694.2018.00.194. doi: 10.3969/j.issn.1002-2694.2018.00.194
[16]	Kasempimolporn S, Thaveekarn W, Promrungreang K, et al. Improved serodiagnostic sensitivity of strip test for latent tuberculosis. J Clin Diagn Res, 2017, 11(6): DC01-DC03. doi: 10.7860/JCDR/2017/25860.9994. doi: 10.7860/JCDR/2017/25860.9994
[17]	Kruh-Garcia NA, Wolfe LM, Dobos KM. Deciphering the role of exosomes in tuberculosis. Tuberculosis (Edinb), 2015, 95(1):26-30. doi: 10.1016/j.tube.2014.10.010. doi: 10.1016/j.tube.2014.10.010 URL
[18]	Ma G, Pan J, Han J, et al. Identification of M.tuberculosis antigens in the sera of tuberculosis patients using biomimetic affinity chromatography in conjunction with ESI-CID-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci, 2017, 1061-1062: 452-458. doi: 10.1016/j.jchromb.2017.07.052. doi: 10.1016/j.jchromb.2017.07.052 URL
[19]	Cao T, Lyu L, Jia H, et al. A two-way proteome microarray strategy to identify novel Mycobacterium tuberculosis-human interactions. Front Cell Infect Microbiol, 2019, 9:65. doi: 10.3389/fcimb.2019.00065. doi: 10.3389/fcimb.2019.00065 URL
[20]	张昆南, 许自强, 王朝东, 等. 脑脊液中检测结核分枝杆菌特异性抗原对结核性脑膜炎的诊断意义. 中华神经科杂志, 2011, 44(2):86-90. doi: 10.3760/cma.j.issn.1006-7876.2011.02.004. doi: 10.3760/cma.j.issn.1006-7876.2011.02.004
[21]	Broger T, Tsionksy M, Mathew A, et al. Sensitive electrochemiluminescence (ECL) immunoassays for detecting lipoarabinomannan (LAM) and ESAT-6 in urine and serum from tuberculosis patients. PLoS One, 2019, 14(4):e0215443. doi: 10.1371/journal.pone.0215443. doi: 10.1371/journal.pone.0215443 URL
[22]	王雪梅, 周松林, 熊国亮. 时间分辨荧光分析法测定CFP10对结核性胸腔积液的临床诊断价值. 江西医药, 2017, 52(8):746-749. doi: 10.3969/j.issn.1006-2238.2017.08.011. doi: 10.3969/j.issn.1006-2238.2017.08.011
[23]	崔光辉. 斑点金免疫渗滤试验检价测血清结核分枝杆菌IgG在肺结核诊断中的价值. 医学信息, 2015(17):193-194. doi: 10.3969/j.issn.1006-1959.2015.17.262. doi: 10.3969/j.issn.1006-1959.2015.17.262
[24]	Fan J, Zhang H, Nguyen DT, et al. Rapid diagnosis of new and relapse tuberculosis by quantification of a circulating antigen in HIV-infected adults in the Greater Houston metropolitan area. BMC Med, 2017, 15(1):188. doi: 10.1186/s12916-017-0952-z. doi: 10.1186/s12916-017-0952-z URL
[25]	Szewczyk R, Kowalski K, Janiszewska-Drobinska B, et al. Rapid method for Mycobacterium tuberculosis identification using electrospray ionization tandem mass spectrometry analysis of mycolic acids. Diagn Microbiol Infect Dis, 2013, 76(3):298-305. doi: 10.1016/j.diagmicrobio.2013.03.025. doi: 10.1016/j.diagmicrobio.2013.03.025 URL
[26]	Nicoara SC, Minnikin DE, Lee OC, et al. Development and optimization of a gas chromatography/mass spectrometry method for the analysis of thermochemolytic degradation products of phthiocerol dimycocerosate waxes found in Mycobacterium tuberculosis. Rapid Commun Mass Spectrom, 2013, 27(21):2374-2382. doi: 10.1002/rcm.6694. doi: 10.1002/rcm.6694 URL
[27]	Thakur H, Kaur N, Sareen D, et al. Electrochemical determination of M.tuberculosis antigen based on Poly (3,4-ethylenedioxythiophene) and functionalized carbon nanotubes hybrid platform. Talanta, 2017, 171:115-123. doi: 10.1016/j.talanta.2017.04.063. doi: 10.1016/j.talanta.2017.04.063 URL
[28]	Bai L, Chen Y, Bai Y, et al. Fullerene-doped polyaniline as new redox nanoprobe and catalyst in electrochemical aptasensor for ultrasensitive detection of Mycobacterium tuberculosis MPT64 antigen in human serum. Biomaterials, 2017, 133:11-19. doi: 10.1016/j.biomaterials.2017.04.010. doi: 10.1016/j.biomaterials.2017.04.010 URL
[29]	Chen Y, Liu X, Guo S, et al. A sandwich-type electrochemical aptasensor for Mycobacterium tuberculosis MPT64 antigen detection using C₆₀NPs decorated N-CNTs/GO nanocomposite coupled with conductive PEI-functionalized metal-organic framework. Biomaterials, 2019, 16:119253. doi: 10.1016/j.biomaterials.2019.119253. doi: 10.1016/j.biomaterials.2019.119253